Seawater carbonate chemistry and ROS and EPS production of the Trichodesmium erythraeum

• Main Authors: Wu, Shijie, Mi, Tiezhu, Zhen, Yu, Yu, Elizabeth K, Wang, Fuwen, Yu, Zhigang, Mock, Timothy D
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2021
• Subjects: Bacteria; Bottles or small containers/Aquaria <20 L; Cyanobacteria; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Other studied parameter or process; Pelagos; Phytoplankton; Single species; Trichodesmium erythraeum; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment duration; Treatment; Superoxide dismutase activity, unit per protein mass; Catalase activity, per protein mass; Salinity; Temperature, water; Reactive oxygen species; Reactive oxygen species, standard deviation; Exopolysaccharides; Exopolysaccharides, standard deviation; Growth rate; Growth rate, standard deviation; Chlorophyll a per cell; Chlorophyll a, standard deviation; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Carbon, organic, particulate, per cell; Carbon, organic, particulate, standard deviation; Particulate organic nitrogen per cell; Nitrogen, organic, particulate, per cell, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.930305; https://doi.pangaea.de/10.1594/PANGAEA.930305

The diazotrophic cyanobacterium Trichodesmium is thought to be a major contributor to the new N in the parts of the oligotrophic, subtropical and tropical oceans. In this study physiological and biochemical methods and transcriptome sequencing were used to investigate the influences of ocean acidification (OA) on Trichodesmium erythraeum (T. erythraeum). We presented evidence that OA caused by CO2 slowed the growth rate and physiological activity of T. erythraeum. OA led to reduced development of proportion of the vegetative cells into diazocytes which included up‐regulated genes of nitrogen fixation. Reactive oxygen species (ROS) accumulation was increased due to the disruption of photosynthetic electron transport and decrease in antioxidant enzyme activities under acidified conditions. This study showed that OA increased the amounts of (exopolysaccharides) EPS in T. erythraeum, and the key genes of ribose‐5‐phosphate (R5P) and glycosyltransferases (Tery_3818) were up‐regulated. These results provide new insight into how ROS and EPS of T. erythraeum increase in an acidified future ocean to cope with OA‐imposed stress. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-04-07.