Seawater carbonate chemistry and antioxidant defences, oxidative stress of two marine copepods

• Main Authors: Engström-Öst, Jonna, Kanerva, Mirella, Vuori, Kristiina, Riebesell, Ulf, Spisla, Carsten, Glippa, Olivier
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2020
• Subjects: Animalia; Arthropoda; Calanus finmarchicus; Coast and continental shelf; Field experiment; Mesocosm or benthocosm; North Atlantic; Other studied parameter or process; Pelagos; Single species; Temora longicornis; Temperate; Zooplankton; Event label; Type; Species; Registration number of species; Uniform resource locator/link to reference; DATE/TIME; Mesocosm label; Treatment; Glutathione S-transferase, activity per protein mass; Glutathione reductase, per protein mass; Superoxide dismutase activity, inhibition; Lipid peroxidation, per protein; Catalase activity, per protein mass; Glutathione, total, per protein mass; Glutathione reduced/Glutathione oxidized, ratio; Oxygen reactive absorbance capacity; Total glutathione peroxidases activity, per protein mass; Oxygen reactive absorbance capacity/lipid peroxidation, ratio; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Chlorophyll a; Chlorophyll a, standard deviation; Carbonate system computation flag; pH; pH, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Mesocosm experiment; Potentiometric titration; Calculated using seacarb; Calculated using seacarb after Nisumaa et al. 2010; Calculated using seacarb after Orr et al. 2018; KOSMOS_2015; Ocean Acidification International Coordination Centre OA-ICC; Bergen; Ocean acidification; Copepods
• Online Access: https://dx.doi.org/10.1594/pangaea.926955; https://doi.pangaea.de/10.1594/PANGAEA.926955

We collected samples for oxidative stress and antioxidants in a high CO2 mesocosm experiment for two weeks, focussing on two common crustacean copepods Calanus finmarchicus and Temora longicornis. The samples were collected during a field experiment campaign studying responses of plankton communities to future ocean acidification (OA), off the Norwegian coast south of Bergen. The main results showed that there were species-specific differences between Temora and Calanus, especially in antioxidant defences (glutathione system) and oxidative stress (lipid peroxidation and reduced:oxidised glutathione ratio). Regular monitoring of chlorophyll a and jellyfish abundances taking place during the field campaign revealed that both chl a and predators may have affected the eco-physiological response. Antioxidant and oxidative stress levels are known to respond sensitively to both the food quality and quantity and the predator pressure, apart from environmental (i.e., abiotic) changes. Calanus was more robust towards OA, perhaps due to its high tolerance to a wide range of vertical physical-chemical conditions. Both top-down and bottom-up factors seem to play a role for the outcome of copepod responses to future ocean acidification. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-01-25.