Planktonic foraminifera eDNA signature deposited on the seafloor remains preserved after burial in marine sediments ...

Environmental DNA (eDNA) metabarcoding of marine sediments has revealed large amounts of sequences assigned to planktonic taxa. How this planktonic eDNA is delivered on the seafloor and preserved in the sediment is not well understood. We address these questions by comparing metabarcoding and microf...

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Bibliographic Details
Main Authors: Barrenechea Angeles, Ines, Lejzerowicz, Franck, Cordier, Tristan, Scheplitz, Janin, Kucera, Michal, Ariztegui, Daniel, Pawlowski, Jan, Morard, Raphael
Format: Article in Journal/Newspaper
Language:English
Published: PANGAEA 2020
Subjects:
Foraminifera
Metabarcoding
microfossils
North Atlantic
sedimentary ancient DNA
Center for Marine Environmental Sciences MARUM
Newfoundland
Planktonic foraminifera
Online Access:https://dx.doi.org/10.1594/pangaea.923417
https://doi.pangaea.de/10.1594/PANGAEA.923417
Description
Summary:Environmental DNA (eDNA) metabarcoding of marine sediments has revealed large amounts of sequences assigned to planktonic taxa. How this planktonic eDNA is delivered on the seafloor and preserved in the sediment is not well understood. We address these questions by comparing metabarcoding and microfossil foraminifera assemblages in sediment cores taken off Newfoundland across a strong ecological gradient. We detected planktonic foraminifera eDNA down to 30 cm and observed that the planktonic/benthic amplicon ratio changed with depth. The relative proportion of planktonic foraminiferal amplicons remained low from the surface down to 10 cm, likely due to the presence of DNA from living benthic foraminifera. Below 10 cm, the relative proportion of planktonic foraminifera amplicons rocketed, likely reflecting the higher proportion of planktonic eDNA in the DNA burial flux. In addition, the microfossil and metabarcoding assemblages showed a congruent pattern indicating that planktonic foraminifera eDNA is ...

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