Seawater carbonate chemistry and expression of c-Fos protein in the brain of marine medaka

• Main Authors: Wang, Xiaojie, Lv, Yutao, Xie, Jinling, Li, Baolin, Zhou, Tangjian, Chen, Yaqi, Chen, Yi, Song, Jiakun
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2020
• Subjects: Animalia; Behaviour; Bottles or small containers/Aquaria <20 L; Chordata; Coast and continental shelf; Gene expression incl. proteomics; Laboratory experiment; Nekton; North Pacific; Oryzias melastigma; Pelagos; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Experiment duration; Time in minutes; pH; pH, standard deviation; Gene expression; Gene expression, standard deviation; Time point, descriptive; Proportion of time; Proportion, standard deviation; Speed, swimming; Speed, swimming, standard deviation; Salinity; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Temperature, water; Temperature, water, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Calculated using CO2SYS; Potentiometric titration; Potentiometric; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.920009; https://doi.pangaea.de/10.1594/PANGAEA.920009

Altered behaviors have been reported in many marine fish following exposure to high CO2 concentrations. However, the mechanistic link between elevated CO2 and activation of brain regions in fish is unknown. Herein, we examined the relative quantification and location of c-Fos expression in marine medaka following acute (360 min) and short-term (7 d) exposure to CO2-enriched water (1000 ppm and 1800 ppm CO2). In the control and two treatment groups, pH was stable at 8.21, 7.92 and 7.64, respectively. After acute exposure to seawater acidified by enrichment with CO2, there was a clear upregulation of c-Fos protein in the medaka brain (P : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.