Relative hepatic transcript expression of 48 target genes from post-smolt Atlantic salmon subjected to an incremental temperature increase either under normoxia or hypoxia in a tank-based system ...

• Main Authors: Beemelmanns, Anne, Zanuzzo, Fabio S, Xue, Xi, Sandrelli, Rebeccah M, Gamperl, A Kurt, Rise, Matthew L
• Format: Dataset
• Language: English
• Published: PANGAEA 2020
• Subjects: Aquaculture; Climate change; hypoxia; Increasing Temperatures; Transcriptomics; Atlantic salmon
• Online Access: https://dx.doi.org/10.1594/pangaea.913696; https://doi.pangaea.de/10.1594/PANGAEA.913696

In this study, we measured the relative transcript expression of 45 target genes and 3 normalizer genes in liver samples of 120 Atlantic salmon that were subjected to 3 different climate scenarios in a tank-based system.Atlantic salmon were exposed to following treatments: (1) Control (CT) constant temperature of 12°C and 100 % air saturation; (2) Warm&Normoxic (WN) incremental temperature increase (1°C per week from 12 - 20°C) at 100% air saturation; and (3) Warm&Hypoxic (WH) decrease in oxygen content of 70% air saturation over one week, followed by two weeks of acclimation to this oxygen level, and then incremental temperature increase (1°C per week from 12 - 20°C) at 70% air saturation. Liver samples were taken from 8 fish per treatment group (CT, WN and WH) at 5 different temperature measuring points: 12°C, 16°C, 18°C, 19°C, and 20°C. For each treatment group, we used 8 biological fish replicates (4 fish from 2 tank replicates). The sampling during the simulated seasonal temperature increase was ...