Microbial cell numbers measured during POLARSTERN cruise PS94 (TransArcII) to the Barents Sea and central Arctic ...

• Main Author: Bienhold, Christina
• Format: Dataset
• Language: English
• Published: PANGAEA 2019
• Subjects: Barents Sea; Central Arctic Ocean; Climate change; microbial abundance; organic-matter; Sediment oxygen uptake; Event label; Date/Time of event; Latitude of event; Longitude of event; Elevation of event; DEPTH, sediment/rock; Depth, top/min; Depth, bottom/max; Prokaryotes, abundance as single cells; Comment; MultiCorer; Giant box grab; Giant box corer; Acridine Orange Direct Counting AODC; PS94; Polarstern; Assessment of bacterial life and matter cycling in deep-sea surface sediments ABYSS; System Laptev-Sea Transdrift TRANSDRIFT; Arctic Ocean; laptev; Laptev Sea
• Online Access: https://dx.doi.org/10.1594/pangaea.907884; https://doi.pangaea.de/10.1594/PANGAEA.907884

For the determination of prokaryotic cell numbers, 1 cm³ sediment was fixed with sterile filtered formalin/seawater at a final concentration of 2% and stored at 4°C until further analyses in the home laboratory. Samples were filtered on 0.2 µm polycarbonate filters, stained with acridine orange (Meyer-Reil, 1983, doi:10.1007/bf00395813), and counted using an epifluorescence microscope (Axiophot II Imaging, Zeiss, Jena, Germany). For each sample, 30 random grids from two replicate filters were counted and averaged. ...