Microbial cell numbers measured during POLARSTERN cruise PS94 (TransArcII) to the Barents Sea and central Arctic ...

For the determination of prokaryotic cell numbers, 1 cm³ sediment was fixed with sterile filtered formalin/seawater at a final concentration of 2% and stored at 4°C until further analyses in the home laboratory. Samples were filtered on 0.2 µm polycarbonate filters, stained with acridine orange (Mey...

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Bibliographic Details
Main Author: Bienhold, Christina
Format: Dataset
Language:English
Published: PANGAEA 2019
Subjects:
Online Access:https://dx.doi.org/10.1594/pangaea.907884
https://doi.pangaea.de/10.1594/PANGAEA.907884
Description
Summary:For the determination of prokaryotic cell numbers, 1 cm³ sediment was fixed with sterile filtered formalin/seawater at a final concentration of 2% and stored at 4°C until further analyses in the home laboratory. Samples were filtered on 0.2 µm polycarbonate filters, stained with acridine orange (Meyer-Reil, 1983, doi:10.1007/bf00395813), and counted using an epifluorescence microscope (Axiophot II Imaging, Zeiss, Jena, Germany). For each sample, 30 random grids from two replicate filters were counted and averaged. ...