Microbial cell numbers measured during POLARSTERN cruise PS94 (TransArcII) to the Barents Sea and central Arctic ...

For the determination of prokaryotic cell numbers, 1 cm³ sediment was fixed with sterile filtered formalin/seawater at a final concentration of 2% and stored at 4°C until further analyses in the home laboratory. Samples were filtered on 0.2 µm polycarbonate filters, stained with acridine orange (Mey...

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Bibliographic Details
Main Author: Bienhold, Christina
Format: Dataset
Language:English
Published: PANGAEA 2019
Subjects:
Barents Sea
Central Arctic Ocean
Climate change
microbial abundance
organic-matter
Sediment oxygen uptake
Event label
Date/Time of event
Latitude of event
Longitude of event
Elevation of event
DEPTH, sediment/rock
Depth, top/min
Depth, bottom/max
Prokaryotes, abundance as single cells
Comment
MultiCorer
Giant box grab
Giant box corer
Acridine Orange Direct Counting AODC
PS94
Polarstern
Assessment of bacterial life and matter cycling in deep-sea surface sediments ABYSS
System Laptev-Sea Transdrift TRANSDRIFT
Arctic Ocean
laptev
Laptev Sea
Online Access:https://dx.doi.org/10.1594/pangaea.907884
https://doi.pangaea.de/10.1594/PANGAEA.907884
Description
Summary:For the determination of prokaryotic cell numbers, 1 cm³ sediment was fixed with sterile filtered formalin/seawater at a final concentration of 2% and stored at 4°C until further analyses in the home laboratory. Samples were filtered on 0.2 µm polycarbonate filters, stained with acridine orange (Meyer-Reil, 1983, doi:10.1007/bf00395813), and counted using an epifluorescence microscope (Axiophot II Imaging, Zeiss, Jena, Germany). For each sample, 30 random grids from two replicate filters were counted and averaged. ...

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