Seawater carbonate chemistry and behavioural abnormalities in coral reef fish, supplement to: Jarrold, Michael; Humphrey, Craig; McCormick, Mark I; Munday, Philip L (2017): Diel CO2 cycles reduce severity of behavioural abnormalities in coral reef fish under ocean acidification. Scientific Reports, 7(1)

• Main Authors: Jarrold, Michael, Humphrey, Craig, McCormick, Mark I, Munday, Philip L
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2017
• Subjects: Acanthochromis polyacanthus; Amphiprion percula; Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Nekton; Pelagos; Single species; South Pacific; Tropical; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment; Identification; Treatment; Individuals; Lateralization; Number of observations; Time; pH; pH, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; McCormick; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.893703; https://doi.pangaea.de/10.1594/PANGAEA.893703

Elevated CO2 levels associated with ocean acidification (OA) have been shown to alter behavioural responses in coral reef fishes. However, all studies to date have used stable pCO2 treatments, not considering the substantial diel pCO2 variation that occurs in shallow reef habitats. Here, we reared juvenile damselfish, Acanthochromis polyacanthus, and clownfish, Amphiprion percula, at stable and diel cycling pCO2 treatments in two experiments. As expected, absolute lateralization of A. polyacanthus and response to predator cue of Am. percula were negatively affected in fish reared at stable, elevated pCO2 in both experiments. However, diel pCO2 fluctuations reduced the negative effects of OA on behaviour. Importantly, in experiment two, behavioural abnormalities that were present in fish reared at stable 750 µatm CO2 were largely absent in fish reared at 750 ± 300 µatm CO2. Overall, we show that diel pCO2 cycles can substantially reduce the severity of behavioural abnormalities caused by elevated CO2. Thus, past studies may have over-estimated the impacts of OA on the behavioural performance of coral reef fishes. Furthermore, our results suggest that diel pCO2 cycles will delay the onset of behavioural abnormalities in natural populations. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-09-04.