Seawater carbonate chemistry and gene abundance and community composition in two contrasting coastal marine sediments ...

• Main Authors: Currie, Ashleigh R, Tait, Karen, Parry, Helen E, de Francisco-Mora, Beatriz, Hicks, Natalie, Osborn, A M, Widdicombe, Steve, Stahl, Henrik
• Format: Dataset
• Language: English
• Published: PANGAEA 2017
• Subjects: Benthos; Coast and continental shelf; Community composition and diversity; Containers and aquaria 20-1000 L or < 1 m**2; Entire community; Laboratory experiment; North Atlantic; Soft-bottom community; Temperate; Temperature; Event label; Type; Experiment duration; Depth, description; Campaign; Identification; Day of experiment; Replicate; Temperature, water; Partial pressure of carbon dioxide water at sea surface temperature wet air; Gene abundance; Species richness; Margelf index; Shannon Diversity Index; Evenness of species; Sequence abundance; Sequence abundance, standard deviation; Salinity; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate system computation flag; pH; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Experiment week; Ammonium; Ammonium, standard deviation; Nitrate and Nitrite; Nitrate and Nitrite, standard deviation; Phosphate; Phosphate, standard deviation; Experiment; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.890872; https://doi.pangaea.de/10.1594/PANGAEA.890872

Marine ecosystems are exposed to a range of human-induced climate stressors, in particular changing carbonate chemistry and elevated sea surface temperatures as a consequence of climate change. More research effort is needed to reduce uncertainties about the effects of global-scale warming and acidification for benthic microbial communities, which drive sedimentary biogeochemical cycles. In this research, mesocosm experiments were set up using muddy and sandy coastal sediments to investigate the independent and interactive effects of elevated carbon dioxide concentrations (750 ppm CO2) and elevated temperature (ambient + 4 °C) on the abundance of taxonomic and functional microbial genes. Specific q-PCR primers were used to target archaeal, bacterial and cyanobacterial/chloroplast 16S rRNA in both sediment types. Nitrogen cycling genes archaeal and bacterial ammonia monooxygenase (amoA) and bacterial nitrite reductase (nirS) were specifically targeted to identify changes in microbial gene abundance and ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-05-23. ...