Seawater carbonate chemistry and gene abundance and community composition in two contrasting coastal marine sediments ...

Marine ecosystems are exposed to a range of human-induced climate stressors, in particular changing carbonate chemistry and elevated sea surface temperatures as a consequence of climate change. More research effort is needed to reduce uncertainties about the effects of global-scale warming and acidi...

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Bibliographic Details
Main Authors: Currie, Ashleigh R, Tait, Karen, Parry, Helen E, de Francisco-Mora, Beatriz, Hicks, Natalie, Osborn, A M, Widdicombe, Steve, Stahl, Henrik
Format: Dataset
Language:English
Published: PANGAEA 2017
Subjects:
pH
Online Access:https://dx.doi.org/10.1594/pangaea.890872
https://doi.pangaea.de/10.1594/PANGAEA.890872
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Summary:Marine ecosystems are exposed to a range of human-induced climate stressors, in particular changing carbonate chemistry and elevated sea surface temperatures as a consequence of climate change. More research effort is needed to reduce uncertainties about the effects of global-scale warming and acidification for benthic microbial communities, which drive sedimentary biogeochemical cycles. In this research, mesocosm experiments were set up using muddy and sandy coastal sediments to investigate the independent and interactive effects of elevated carbon dioxide concentrations (750 ppm CO2) and elevated temperature (ambient + 4 °C) on the abundance of taxonomic and functional microbial genes. Specific q-PCR primers were used to target archaeal, bacterial and cyanobacterial/chloroplast 16S rRNA in both sediment types. Nitrogen cycling genes archaeal and bacterial ammonia monooxygenase (amoA) and bacterial nitrite reductase (nirS) were specifically targeted to identify changes in microbial gene abundance and ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-05-23. ...