Seawater carbonate chemistry and immune function of the Pacific oyster against Vibrio splendidus challenge ...

• Main Authors: Cao, Ruiwen, Wang, Qing, Yang, Dinglong, Liu, Yongliang, Ran, Wen, Qu, Yi, Wu, Huifeng, Cong, Ming, Li, Fei, Ji, Chenglong, Zhao, Jianmin
• Format: Dataset
• Language: English
• Published: PANGAEA 2018
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Crassostrea gigas; Immunology/Self-protection; Laboratory experiment; Mollusca; North Pacific; Other; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment duration; Partial pressure of carbon dioxide water at sea surface temperature wet air; Treatment; Phagocytosis rate; Phagocytosis rate, standard deviation; Reactive oxygen species production; Reactive oxygen species production, standard deviation; Percentage; Percentage, standard deviation; Hemocyte count; Hemocyte count, standard deviation; Number of expressed proteins; Name; Group; Fold change; Gene expression; Gene expression, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Pacific; Ocean acidification; Pacific oyster
• Online Access: https://dx.doi.org/10.1594/pangaea.889437; https://doi.pangaea.de/10.1594/PANGAEA.889437

Ocean acidification (OA) and pathogenic diseases pose a considerable threat to key species of marine ecosystem. However, few studies have investigated the combined impact of reduced seawater pH and pathogen challenge on the immune responses of marine invertebrates. In this study, Pacific oysters, Crassostrea gigas, were exposed to OA (~2000 ppm) for 28 days and then challenged with Vibrio splendidus for another 72 h. Hemocyte parameters showed that V. splendidus infection exacerbated the impaired oyster immune responses under OA exposure. An iTRAQ-based quantitative proteomic analysis revealed that C. gigas responded differently to OA stress and V. splendidus challenge, alone or in combination. Generally, OA appears to act via a generalized stress response by causing oxidative stress, which could lead to cellular injury and cause disruption to the cytoskeleton, protein turnover, immune responses and energy metabolism. V. splendidus challenge in oysters could suppress the immune system directly and lead to a ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-05-02. ...