Seawater carbon chemistry and regenerative capacity of in adult sea urchins spines and tube feet

• Main Authors: Emerson, Chloe E, Reinardy, Helena C, Bates, Nicolas R, Bodnar, Andrea G
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2017
• Subjects: Animalia; Behaviour; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Echinodermata; Gene expression incl. proteomics; Growth/Morphology; Laboratory experiment; Lytechinus variegatus; North Atlantic; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Identification; Treatment; Experiment duration; Category; Length; Length, standard error; Mass; Mass, standard error; Magnesium/Calcium ratio; Magnesium/Calcium ratio, standard error; Strontium/Calcium ratio; Strontium/Calcium ratio, standard error; Barium/Calcium ratio; Barium/Calcium ratio, standard error; Lithium/Calcium ratio; Lithium/Calcium ratio, standard error; Incubation duration; Gene expression, fold change, relative; Gene expression, fold change, relative, standard error; Time in seconds; Time, standard error; Percentage; Temperature, water; Temperature, water, standard error; Salinity; Salinity, standard error; Conductivity; Conductivity, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Alkalinity, total; Alkalinity, total, standard error; pH; pH, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Calcite saturation state; Calcite saturation state, standard error; Aragonite saturation state; Aragonite saturation state, standard error; High magnesium calcite saturation state; High magnesium calcite saturation state, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Experiment; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.878254; https://doi.pangaea.de/10.1594/PANGAEA.878254

Increasing atmospheric carbon dioxide (CO2) has resulted in a change in seawater chemistry and lowering of pH, referred to as ocean acidification. Understanding how different organisms and processes respond to ocean acidification is vital to predict how marine ecosystems will be altered under future scenarios of continued environmental change. Regenerative processes involving biomineralization in marine calcifiers such as sea urchins are predicted to be especially vulnerable. In this study, the effect of ocean acidification on regeneration of external appendages (spines and tube feet) was investigated in the sea urchin Lytechinus variegatus exposed to ambient (546 µatm), intermediate (1027 µatm) and high (1841 µatm) partial pressure of CO2 (pCO2) for eight weeks. The rate of regeneration was maintained in spines and tube feet throughout two periods of amputation and regrowth under conditions of elevated pCO2. Increased expression of several biomineralization-related genes indicated molecular compensatory mechanisms; however, the structural integrity of both regenerating and homeostatic spines was compromised in high pCO2 conditions. Indicators of physiological fitness (righting response, growth rate, coelomocyte concentration and composition) were not affected by increasing pCO2, but compromised spine integrity is likely to have negative consequences for defence capabilities and therefore survival of these ecologically and economically important organisms. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-07-19.