Length and width measurements of Fragilariopsis kerguelensis valves from sediment core PS1768-8 ...

• Main Authors: Kloster, Michael, Esper, Oliver, Kauer, Gerhard, Beszteri, Bánk
• Format: Dataset
• Language: English
• Published: PANGAEA 2017
• Subjects: DEPTH, sediment/rock; Sample ID; File name; Fragilariopsis kerguelensis valve, width; Fragilariopsis kerguelensis valve, height; Method comment; Index; Marine isotopic stage; Gravity corer Kiel type; Automated microscopy and image analysis workflow Kloster et al. 2017; ANT-VIII/3; Polarstern; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158; Arctic; Antarctic; Antarc*; Sea ice
• Online Access: https://dx.doi.org/10.1594/pangaea.873993; https://doi.pangaea.de/10.1594/PANGAEA.873993

Light microscopy analysis of diatom frustules is widely used in basic and applied research, notably taxonomy, morphometrics, water quality monitoring and paleo-environmental studies. Although there is a need for automation in these applications, various developments in image processing and analysis methodology supporting these tasks have not become widespread in diatom-based analyses. We have addressed this issue by combining our automated diatom image analysis software SHERPA with a commercial slide-scanning microscope. The resulting workflow enables mass-analyses of a broad range of morphometric features from individual frustules mounted on permanent slides. Extensive automation and internal quality control of the results helps to minimize user intervention, but care was taken to allow the user to stay in control of the most critical steps (exact segmentation of valve outlines and selection of objects of interest) using interactive functions for reviewing and revising results. In this contribution, we ... : Supplement to: Kloster, Michael; Esper, Oliver; Kauer, Gerhard; Beszteri, Bánk (2017): Large scale permanent slide imaging and image analysis for diatom morphometrics. Applied Sciences, 7(4), 330 ...