Effects of short-term hypoxia and seawater acidification on hemocyte responses of the mussel Mytilus coruscus ...

• Main Authors: Sui, Yanming, Kong, Hui, Shang, Yueyong, Huang, Xizhi, Wu, Fangli, Hu, Menghong, Lin, Daohui, Lu, Weiqun, Wang, Youji
• Format: Dataset
• Language: English
• Published: PANGAEA 2016
• Subjects: Animalia; Benthic animals; Benthos; Brackish waters; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Mollusca; Mytilus coruscus; North Pacific; Other metabolic rates; Oxygen; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Time in hours; pH; Hemocyte mortality; Percentage; Reactive oxygen species production; Lysosomal content; Hemocyte count; Temperature, water; Oxygen, standard deviation; Salinity; Salinity, standard deviation; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Experiment; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.872313; https://doi.pangaea.de/10.1594/PANGAEA.872313

Hypoxia often intensifies with rising dissolved CO2, but the concurrent effects of hypoxia and acidification on bivalves are largely unknown. In this study, immune responses of hemocytes in the mussel Mytilus coruscus were examined under six combinations of pH (7.3, 7.7 and 8.1) and dissolved oxygen (DO) concentrations (2 mg/ L, 6 mg/L) for 72 h. Generally, total hemocyte account, phagocytosis, esterase and lysosomal content were reduced under low DO and pH conditions, whereas hemocyte mortality and reactive oxygen species production increased under low DO and pH. Both hypoxia and low pH have negative effects on mussels, but the effects of pH are not as strong as DO. Moreover, significant interactions between DO and pH occurred. However, acidification generally doesn't aggravate the effects induced by hypoxia. Acidification and hypoxia may increase disease risk and impact the aquaculture of this species. ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-02-16. ...