Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis) ...

• Main Authors: Hernroth, Bodil, Baden, Susanne, Tassidis, H, Hörnaeus, K, Guillemant, J, Bergström Lind, S, Bergquist, Jonas
• Format: Dataset
• Language: English
• Published: PANGAEA 2016
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus edulis; North Atlantic; Polar; Single species; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Growth inhibition; Temperature, water; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.868737; https://doi.pangaea.de/10.1594/PANGAEA.868737

Here, we aimed to investigate potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, as gills are directly facing seawater and the changing pH (predicted to be reduced from 8.1 to 7.7 by 2100). The AMP activity of gill and haemocyte extracts was compared at pH 6.0, 7.7 and 8.1, with a radial diffusion assay against Escherichia coli. The activity of the gill extracts was not affected by pH, while it was significantly reduced with increasing pH in the haemocyte extracts. Gill extracts were also tested against different species of Vibrio (V. parahaemolyticus Vibrio tubiashii, V. splendidus and V. alginoyticus) at pH 7.7 and 8.1. The metabolic activity of the bacteria decreased by 65-90%, depending on species of bacteria, but was, as in the radial diffusion assay, not affected by pH. The results indicated that AMPs from gills are efficient in a broad pH-range. However, when mussels were pre-exposed for pH 7.7 for four month the gill extracts presented ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-11-22. ...