Decreased photosynthesis and growth with reduced respiration in the model diatom Phaeodactylum tricornutum grown under elevated CO2 over 1800 generations, supplement to: Li, Futian; Beardall, John; Collins, Sinéad; Gao, Kunshan (2016): Decreased photosynthesis and growth with reduced respiration in the model diatom Phaeodactylum tricornutum grown under elevated CO2 over 1800 generations. Global Change Biology

• Main Authors: Li, Futian, Beardall, John, Collins, Sinéad, Gao, Kunshan
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2017
• Subjects: Bottles or small containers/Aquaria <20 L; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Type; Species; Registration number of species; Uniform resource locator/link to reference; Figure; Treatment; Time in days; Ratio; Ratio, standard deviation; Growth rate; Growth rate, standard deviation; Cell size; Cell size, standard deviation; Chlorophyll a per cell; Chlorophyll a, standard deviation; Carotenoids per cell; Carotenoids, standard deviation; Respiration rate, oxygen, per chlorophyll a; Respiration rate, oxygen, standard deviation; Respiration rate, oxygen, per cell; Net photosynthesis rate, oxygen, per chlorophyll a; Net photosynthesis rate, standard deviation; Net photosynthesis rate, oxygen, per cell; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Effective photochemical quantum yield; Effective photochemical quantum yield, standard deviation; Non photochemical quenching; Non photochemical quenching, standard deviation; Electron transport rate efficiency; Electron transport rate efficiency, standard deviation; Maximal electron transport rate, relative; Maximal electron transport rate, relative, standard deviation; Light saturation point; Light saturation point, standard deviation; Relative photoinhibition ratio; Relative photoinhibition ratio, standard deviation; Salinity; Temperature, water; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.867319; https://doi.pangaea.de/10.1594/PANGAEA.867319

Studies on the long-term responses of marine phytoplankton to ongoing ocean acidification (OA) are appearing rapidly in the literature. However, only a few of these have investigated diatoms, which is disproportionate to their contribution to global primary production. Here we show that a population of the model diatom Phaeodactylum tricornutum, after growing under elevated CO2 (1000 matm, HCL, pHT: 7.70) for 1860 generations, showed significant differences in photosynthesis and growth from a population maintained in ambient CO2 and then transferred to elevated CO2 for 20 generations (HC). The HCL population had lower mitochondrial respiration, than did the control population maintained in ambient CO2 (400 matm, LCL, pHT: 8.02) for 1860 generations. Although the cells had higher respiratory carbon loss within 20 generations under the elevated CO2, being consistent to previous findings, they down-regulated their respiration to sustain their growth in longer duration under the OA condition. Responses of phytoplankton to OA may depend on the timescale for which they are exposed due to fluctuations in physiological traits over time. This study provides the first evidence that populations of the model species, P. tricornutum, differ phenotypically from each other after having been grown for differing spans of time under OA conditions, suggesting that long-term changes should be measured to understand responses of primary producers to OA, especially in waters with diatom-dominated phytoplankton assemblages. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-10-24.