Ocean acidification weakens the immune response of blood clam through hampering the NF-kappa beta and toll-like receptor pathways ...

• Main Authors: Liu, Saixi, Shi, Wei, Guo, Cheng, Zhao, Xinguo, Han, Yu, Peng, Chao, Chai, Xueliang, Liu, Guangxu
• Format: Dataset
• Language: English
• Published: PANGAEA 2016
• Subjects: Animalia; Benthic animals; Benthos; Brackish waters; Containers and aquaria 20-1000 L or < 1 m**2; Gene expression incl. proteomics; Growth/Morphology; Immunology/Self-protection; Laboratory experiment; Mollusca; North Pacific; Single species; Tegillarca granosa; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Height; Height, standard error; Tissues; Date; Treatment; Gene name; mRNA gene expression, relative; mRNA gene expression, relative, standard error; Hemocyte count; Hemocyte count, standard error; Percentage; Percentage, standard error; Phagocytosis rate; Phagocytosis rate, standard error; pH; pH, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Salinity; Salinity, standard error; Temperature, water; Temperature, water, standard error; Alkalinity, total; Alkalinity, total, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Aragonite saturation state; Aragonite saturation state, standard error; Calcite saturation state; Calcite saturation state, standard error; Carbonate system computation flag; Carbon dioxide; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.861971; https://doi.pangaea.de/10.1594/PANGAEA.861971

The impact of pCO2 driven ocean acidification on marine bivalve immunity remains poorly understood. To date, this impact has only been investigated in a few bivalve species and the underlying molecular mechanism remains unknown. In the present study, the effects of the realistic future ocean pCO2 levels (pH at 8.1, 7.8, and 7.4) on the total number of haemocyte cells (THC), phagocytosis status, blood cell types composition, and expression levels of twelve genes from the NF-kappa beta signaling and toll-like receptor pathways of a typical bottom burrowing bivalve, blood clam (Tegillarca granosa), were investigated. The results obtained showed that while both THC number and phagocytosis frequency were significantly reduced, the percentage of red and basophil granulocytes were significantly decreased and increased, respectively, upon exposure to elevated pCO2. In addition, exposure to pCO2 acidified seawater generally led to a significant down-regulation in the inducer and key response genes of NF-kappa beta ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-06-21. ...