Response of spring diatoms to CO2 availability in the Western North Pacific as determined by next-generation sequencing ...

• Main Authors: Endo, H, Sugie, Koji, Yoshimura, T, Suzuki, Koji
• Format: Dataset
• Language: English
• Published: PANGAEA 2016
• Subjects: Community composition and diversity; Containers and aquaria 20-1000 L or < 1 m**2; Entire community; Growth/Morphology; Laboratory experiment; North Pacific; Open ocean; Pelagos; Temperate; Type; Treatment; Incubation duration; Growth rate; Growth rate, standard deviation; Group; Percentage; rbcL gene, copy number; rbcL gene, copy number, standard deviation; rbcL transcript, copy number; rbcL transcript, copy number, standard deviation; Taxon/taxa; Contribution; Chlorophyll a; Chlorophyll a, standard deviation; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Operational taxonomic unit; Shannon Diversity Index; Confidence interval; Simpson index of diversity; Temperature, water; Salinity; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; pH; pH, standard deviation; Nitrate; Nitrate, standard deviation; Phosphate; Phosphate, standard deviation; Silicate; Silicate, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.861965; https://doi.pangaea.de/10.1594/PANGAEA.861965

Next-generation sequencing (NGS) technologies have enabled us to determine phytoplankton community compositions at high resolution. However, few studies have adopted this approach to assess the responses of natural phytoplankton communities to environmental change. Here, we report the impact of different CO2 levels on spring diatoms in the Oyashio region of the western North Pacific as estimated by NGS of the diatom-specific rbcL gene (DNA), which encodes the large subunit of RubisCO. We also examined the abundance and composition of rbcL transcripts (cDNA) in diatoms to assess their physiological responses to changing CO2 levels. A short-term (3-day) incubation experiment was carried out on-deck using surface Oyashio waters under different pCO2 levels (180, 350, 750, and 1000 µatm) in May 2011. During the incubation, the transcript abundance of the diatom-specific rbcL gene decreased with an increase in seawater pCO2 levels. These results suggest that CO2 fixation capacity of diatoms decreased rapidly under ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-06-21. ...