Impact of CO2 enrichment on organic matter dynamics - Enrichment experiment

• Main Author: Engel, Anja
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Experimental treatment; Bacteria; Bacteria, abundance, standard deviation; Leucine aminopeptidase activity, cell normalized; Leucine aminopeptidase activity, standard deviation; Biological Impacts of Ocean Acidification BIOACID; Surface Ocean Processes in the Anthropocene SOPRAN; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.861366; https://doi.pangaea.de/10.1594/PANGAEA.861366

Cells were incubated for 3 days at in-situ temperature and in the dark. Incubations were analysed for bacterial abundances by flow cytometry after nucleic acid staining with Sybr Green I (Invitrogen). For determination of LAP activities, L-leucyl-4-methylcoumarinyl-7-amide was added to final concentrations of 50 µmo/L. The initial abundance of free-living bacteria was 2.0 x 10**5 cells/ml, representing about 20% of the total bacterioplankton community.