Impact of CO2 enrichment on organic matter dynamics - Enrichment experiment

Cells were incubated for 3 days at in-situ temperature and in the dark. Incubations were analysed for bacterial abundances by flow cytometry after nucleic acid staining with Sybr Green I (Invitrogen). For determination of LAP activities, L-leucyl-4-methylcoumarinyl-7-amide was added to final concent...

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Bibliographic Details
Main Author: Engel, Anja
Format: Dataset
Language:English
Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
Subjects:
Experimental treatment
Bacteria
Bacteria, abundance, standard deviation
Leucine aminopeptidase activity, cell normalized
Leucine aminopeptidase activity, standard deviation
Biological Impacts of Ocean Acidification BIOACID
Surface Ocean Processes in the Anthropocene SOPRAN
Ocean acidification
Online Access:https://dx.doi.org/10.1594/pangaea.861366
https://doi.pangaea.de/10.1594/PANGAEA.861366
Description
Summary:Cells were incubated for 3 days at in-situ temperature and in the dark. Incubations were analysed for bacterial abundances by flow cytometry after nucleic acid staining with Sybr Green I (Invitrogen). For determination of LAP activities, L-leucyl-4-methylcoumarinyl-7-amide was added to final concentrations of 50 µmo/L. The initial abundance of free-living bacteria was 2.0 x 10**5 cells/ml, representing about 20% of the total bacterioplankton community.

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