Community structure of sulfate reducing bacteria in the sediment of the arctic Smeerenburgfjorden ...

• Main Authors: Ravenschlag, Katrin, Sahm, Kerstin, Knoblauch, Christian, Jørgensen, Bo Barker, Amann, Rudolf I
• Format: Dataset
• Language: English
• Published: PANGAEA 2016
• Subjects: Event label; Date/Time of event; Latitude of event; Longitude of event; DEPTH, sediment/rock; Prokaryotes, abundance as single cells; Bacteria, targed with EUB338I-III oligonucleotide FISH-probe; Desulfovibrio spp., targeted with DSV698 oligonucleotides FISH-probe; Desulforhopalus spp., targeted with DSR651 oligonucleotides FISH-probe; Desulfobulbus spp., targeted with 660 oligonucleotides FISH-probe; Desulfotalea spp., targeted with Sval428 oligonucleotides FISH-probe; Desulfobacter spp., targeted with DSB985 oligonucleotides FISH-probe; Desulfobacterium spp., targeted with 221 oligonucleotides FISH-probe; Desulfusarcina/Desulfococcus, targeted with DSS658 oligonucleotide FISH-probe; Bacteria, sulfate reducing; Svalbard clone group SVAL1, targeted with DSS225 oligonucleotide FISH-probe; Svalbard clones Sva0081/Sva0863, targeted with cl81-644 oligonucleotide FISH-probe; Desulfuromonas spp., targeted with DRM432 oligonucleotides FISH-probe; Core; Epifluorescence microscopy after DAPI staining; Fluorescence in situ hybridization FISH; Arctic; Smeerenburgfjorden; Svalbard; Smeerenburgfjord*
• Online Access: https://dx.doi.org/10.1594/pangaea.858334; https://doi.pangaea.de/10.1594/PANGAEA.858334

The community structure of sulfate-reducing bacteria (SRB) of a marine Arctic sediment (Smeerenburgfjorden, Svalbard) was characterized by both fluorescence in situ hybridization (FISH) by using group- and genus-specific 16S rRNA-targeted oligonucleotide probes. Samples stored in PBS-ethanol were diluted and treated by mild sonication. A 10-ml aliquot of a 1:40 dilution was filtered onto a 0.2-mm-pore-size type GTTP polycarbonate filter (Millipore, Eschborn, Germany). Hybridization and microscopic counting of hybridized and 49,69-diamidino-2-phenylindole (DAPI)-stained cells were performed as described previously from Snaidr et al. (1997, http://aem.asm.org/content/63/7/2884.full.pdf). Details of probes and formamide concentrations which were used are listed in futher details.. Means were calculated by using 10 to 20 randomly chosen fields for each filter section, which corresponded to 800 to 1,000 DAPI-stained cells. Counting results were always corrected by subtracting signals observed with probe NON338. ... : Supplement to: Ravenschlag, Katrin; Sahm, Kerstin; Knoblauch, Christian; Jørgensen, Bo Barker; Amann, Rudolf I (2000): Community Structure, Cellular rRNA Content, and Activity of Sulfate-Reducing Bacteria in Marine Arctic Sediments. Applied and Environmental Microbiology, 66(8), 3592-3602 ...