Community structure of sulfate reducing bacteria in the sediment of the arctic Smeerenburgfjorden ...
The community structure of sulfate-reducing bacteria (SRB) of a marine Arctic sediment (Smeerenburgfjorden, Svalbard) was characterized by both fluorescence in situ hybridization (FISH) by using group- and genus-specific 16S rRNA-targeted oligonucleotide probes. Samples stored in PBS-ethanol were di...
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Online Access: | https://dx.doi.org/10.1594/pangaea.858334 https://doi.pangaea.de/10.1594/PANGAEA.858334 |
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ftdatacite:10.1594/pangaea.858334 2024-02-27T08:37:45+00:00 Community structure of sulfate reducing bacteria in the sediment of the arctic Smeerenburgfjorden ... Ravenschlag, Katrin Sahm, Kerstin Knoblauch, Christian Jørgensen, Bo Barker Amann, Rudolf I 2016 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.858334 https://doi.pangaea.de/10.1594/PANGAEA.858334 en eng PANGAEA https://store.pangaea.de/Publications/Ravenschlag-etal_2000/Oligonucleotide-probes_Ravenschlag-etal_2000.pdf https://dx.doi.org/10.1128/aem.66.8.3592-3602.2000 https://store.pangaea.de/Publications/Ravenschlag-etal_2000/Oligonucleotide-probes_Ravenschlag-etal_2000.pdf Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 Event label Date/Time of event Latitude of event Longitude of event DEPTH, sediment/rock Prokaryotes, abundance as single cells Bacteria, targed with EUB338I-III oligonucleotide FISH-probe Desulfovibrio spp., targeted with DSV698 oligonucleotides FISH-probe Desulforhopalus spp., targeted with DSR651 oligonucleotides FISH-probe Desulfobulbus spp., targeted with 660 oligonucleotides FISH-probe Desulfotalea spp., targeted with Sval428 oligonucleotides FISH-probe Desulfobacter spp., targeted with DSB985 oligonucleotides FISH-probe Desulfobacterium spp., targeted with 221 oligonucleotides FISH-probe Desulfusarcina/Desulfococcus, targeted with DSS658 oligonucleotide FISH-probe Bacteria, sulfate reducing Svalbard clone group SVAL1, targeted with DSS225 oligonucleotide FISH-probe Svalbard clones Sva0081/Sva0863, targeted with cl81-644 oligonucleotide FISH-probe Desulfuromonas spp., targeted with DRM432 oligonucleotides FISH-probe Core Epifluorescence microscopy after DAPI staining Fluorescence in situ hybridization FISH dataset Supplementary Dataset Dataset 2016 ftdatacite https://doi.org/10.1594/pangaea.85833410.1128/aem.66.8.3592-3602.2000 2024-02-01T16:08:38Z The community structure of sulfate-reducing bacteria (SRB) of a marine Arctic sediment (Smeerenburgfjorden, Svalbard) was characterized by both fluorescence in situ hybridization (FISH) by using group- and genus-specific 16S rRNA-targeted oligonucleotide probes. Samples stored in PBS-ethanol were diluted and treated by mild sonication. A 10-ml aliquot of a 1:40 dilution was filtered onto a 0.2-mm-pore-size type GTTP polycarbonate filter (Millipore, Eschborn, Germany). Hybridization and microscopic counting of hybridized and 49,69-diamidino-2-phenylindole (DAPI)-stained cells were performed as described previously from Snaidr et al. (1997, http://aem.asm.org/content/63/7/2884.full.pdf). Details of probes and formamide concentrations which were used are listed in futher details.. Means were calculated by using 10 to 20 randomly chosen fields for each filter section, which corresponded to 800 to 1,000 DAPI-stained cells. Counting results were always corrected by subtracting signals observed with probe NON338. ... : Supplement to: Ravenschlag, Katrin; Sahm, Kerstin; Knoblauch, Christian; Jørgensen, Bo Barker; Amann, Rudolf I (2000): Community Structure, Cellular rRNA Content, and Activity of Sulfate-Reducing Bacteria in Marine Arctic Sediments. Applied and Environmental Microbiology, 66(8), 3592-3602 ... Dataset Arctic Smeerenburgfjord* Svalbard DataCite Metadata Store (German National Library of Science and Technology) Arctic Smeerenburgfjorden ENVELOPE(11.158,11.158,79.681,79.681) Svalbard |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Event label Date/Time of event Latitude of event Longitude of event DEPTH, sediment/rock Prokaryotes, abundance as single cells Bacteria, targed with EUB338I-III oligonucleotide FISH-probe Desulfovibrio spp., targeted with DSV698 oligonucleotides FISH-probe Desulforhopalus spp., targeted with DSR651 oligonucleotides FISH-probe Desulfobulbus spp., targeted with 660 oligonucleotides FISH-probe Desulfotalea spp., targeted with Sval428 oligonucleotides FISH-probe Desulfobacter spp., targeted with DSB985 oligonucleotides FISH-probe Desulfobacterium spp., targeted with 221 oligonucleotides FISH-probe Desulfusarcina/Desulfococcus, targeted with DSS658 oligonucleotide FISH-probe Bacteria, sulfate reducing Svalbard clone group SVAL1, targeted with DSS225 oligonucleotide FISH-probe Svalbard clones Sva0081/Sva0863, targeted with cl81-644 oligonucleotide FISH-probe Desulfuromonas spp., targeted with DRM432 oligonucleotides FISH-probe Core Epifluorescence microscopy after DAPI staining Fluorescence in situ hybridization FISH |
spellingShingle |
Event label Date/Time of event Latitude of event Longitude of event DEPTH, sediment/rock Prokaryotes, abundance as single cells Bacteria, targed with EUB338I-III oligonucleotide FISH-probe Desulfovibrio spp., targeted with DSV698 oligonucleotides FISH-probe Desulforhopalus spp., targeted with DSR651 oligonucleotides FISH-probe Desulfobulbus spp., targeted with 660 oligonucleotides FISH-probe Desulfotalea spp., targeted with Sval428 oligonucleotides FISH-probe Desulfobacter spp., targeted with DSB985 oligonucleotides FISH-probe Desulfobacterium spp., targeted with 221 oligonucleotides FISH-probe Desulfusarcina/Desulfococcus, targeted with DSS658 oligonucleotide FISH-probe Bacteria, sulfate reducing Svalbard clone group SVAL1, targeted with DSS225 oligonucleotide FISH-probe Svalbard clones Sva0081/Sva0863, targeted with cl81-644 oligonucleotide FISH-probe Desulfuromonas spp., targeted with DRM432 oligonucleotides FISH-probe Core Epifluorescence microscopy after DAPI staining Fluorescence in situ hybridization FISH Ravenschlag, Katrin Sahm, Kerstin Knoblauch, Christian Jørgensen, Bo Barker Amann, Rudolf I Community structure of sulfate reducing bacteria in the sediment of the arctic Smeerenburgfjorden ... |
topic_facet |
Event label Date/Time of event Latitude of event Longitude of event DEPTH, sediment/rock Prokaryotes, abundance as single cells Bacteria, targed with EUB338I-III oligonucleotide FISH-probe Desulfovibrio spp., targeted with DSV698 oligonucleotides FISH-probe Desulforhopalus spp., targeted with DSR651 oligonucleotides FISH-probe Desulfobulbus spp., targeted with 660 oligonucleotides FISH-probe Desulfotalea spp., targeted with Sval428 oligonucleotides FISH-probe Desulfobacter spp., targeted with DSB985 oligonucleotides FISH-probe Desulfobacterium spp., targeted with 221 oligonucleotides FISH-probe Desulfusarcina/Desulfococcus, targeted with DSS658 oligonucleotide FISH-probe Bacteria, sulfate reducing Svalbard clone group SVAL1, targeted with DSS225 oligonucleotide FISH-probe Svalbard clones Sva0081/Sva0863, targeted with cl81-644 oligonucleotide FISH-probe Desulfuromonas spp., targeted with DRM432 oligonucleotides FISH-probe Core Epifluorescence microscopy after DAPI staining Fluorescence in situ hybridization FISH |
description |
The community structure of sulfate-reducing bacteria (SRB) of a marine Arctic sediment (Smeerenburgfjorden, Svalbard) was characterized by both fluorescence in situ hybridization (FISH) by using group- and genus-specific 16S rRNA-targeted oligonucleotide probes. Samples stored in PBS-ethanol were diluted and treated by mild sonication. A 10-ml aliquot of a 1:40 dilution was filtered onto a 0.2-mm-pore-size type GTTP polycarbonate filter (Millipore, Eschborn, Germany). Hybridization and microscopic counting of hybridized and 49,69-diamidino-2-phenylindole (DAPI)-stained cells were performed as described previously from Snaidr et al. (1997, http://aem.asm.org/content/63/7/2884.full.pdf). Details of probes and formamide concentrations which were used are listed in futher details.. Means were calculated by using 10 to 20 randomly chosen fields for each filter section, which corresponded to 800 to 1,000 DAPI-stained cells. Counting results were always corrected by subtracting signals observed with probe NON338. ... : Supplement to: Ravenschlag, Katrin; Sahm, Kerstin; Knoblauch, Christian; Jørgensen, Bo Barker; Amann, Rudolf I (2000): Community Structure, Cellular rRNA Content, and Activity of Sulfate-Reducing Bacteria in Marine Arctic Sediments. Applied and Environmental Microbiology, 66(8), 3592-3602 ... |
format |
Dataset |
author |
Ravenschlag, Katrin Sahm, Kerstin Knoblauch, Christian Jørgensen, Bo Barker Amann, Rudolf I |
author_facet |
Ravenschlag, Katrin Sahm, Kerstin Knoblauch, Christian Jørgensen, Bo Barker Amann, Rudolf I |
author_sort |
Ravenschlag, Katrin |
title |
Community structure of sulfate reducing bacteria in the sediment of the arctic Smeerenburgfjorden ... |
title_short |
Community structure of sulfate reducing bacteria in the sediment of the arctic Smeerenburgfjorden ... |
title_full |
Community structure of sulfate reducing bacteria in the sediment of the arctic Smeerenburgfjorden ... |
title_fullStr |
Community structure of sulfate reducing bacteria in the sediment of the arctic Smeerenburgfjorden ... |
title_full_unstemmed |
Community structure of sulfate reducing bacteria in the sediment of the arctic Smeerenburgfjorden ... |
title_sort |
community structure of sulfate reducing bacteria in the sediment of the arctic smeerenburgfjorden ... |
publisher |
PANGAEA |
publishDate |
2016 |
url |
https://dx.doi.org/10.1594/pangaea.858334 https://doi.pangaea.de/10.1594/PANGAEA.858334 |
long_lat |
ENVELOPE(11.158,11.158,79.681,79.681) |
geographic |
Arctic Smeerenburgfjorden Svalbard |
geographic_facet |
Arctic Smeerenburgfjorden Svalbard |
genre |
Arctic Smeerenburgfjord* Svalbard |
genre_facet |
Arctic Smeerenburgfjord* Svalbard |
op_relation |
https://store.pangaea.de/Publications/Ravenschlag-etal_2000/Oligonucleotide-probes_Ravenschlag-etal_2000.pdf https://dx.doi.org/10.1128/aem.66.8.3592-3602.2000 https://store.pangaea.de/Publications/Ravenschlag-etal_2000/Oligonucleotide-probes_Ravenschlag-etal_2000.pdf |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_doi |
https://doi.org/10.1594/pangaea.85833410.1128/aem.66.8.3592-3602.2000 |
_version_ |
1792044701783687168 |