Community structure of sulfate reducing bacteria in the sediment of the arctic Smeerenburgfjorden ...
The community structure of sulfate-reducing bacteria (SRB) of a marine Arctic sediment (Smeerenburgfjorden, Svalbard) was characterized by both fluorescence in situ hybridization (FISH) by using group- and genus-specific 16S rRNA-targeted oligonucleotide probes. Samples stored in PBS-ethanol were di...
Main Authors: | , , , , |
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Format: | Dataset |
Language: | English |
Published: |
PANGAEA
2016
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Subjects: | |
Online Access: | https://dx.doi.org/10.1594/pangaea.858334 https://doi.pangaea.de/10.1594/PANGAEA.858334 |
Summary: | The community structure of sulfate-reducing bacteria (SRB) of a marine Arctic sediment (Smeerenburgfjorden, Svalbard) was characterized by both fluorescence in situ hybridization (FISH) by using group- and genus-specific 16S rRNA-targeted oligonucleotide probes. Samples stored in PBS-ethanol were diluted and treated by mild sonication. A 10-ml aliquot of a 1:40 dilution was filtered onto a 0.2-mm-pore-size type GTTP polycarbonate filter (Millipore, Eschborn, Germany). Hybridization and microscopic counting of hybridized and 49,69-diamidino-2-phenylindole (DAPI)-stained cells were performed as described previously from Snaidr et al. (1997, http://aem.asm.org/content/63/7/2884.full.pdf). Details of probes and formamide concentrations which were used are listed in futher details.. Means were calculated by using 10 to 20 randomly chosen fields for each filter section, which corresponded to 800 to 1,000 DAPI-stained cells. Counting results were always corrected by subtracting signals observed with probe NON338. ... : Supplement to: Ravenschlag, Katrin; Sahm, Kerstin; Knoblauch, Christian; Jørgensen, Bo Barker; Amann, Rudolf I (2000): Community Structure, Cellular rRNA Content, and Activity of Sulfate-Reducing Bacteria in Marine Arctic Sediments. Applied and Environmental Microbiology, 66(8), 3592-3602 ... |
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