A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae ...
The cellular mechanisms of calcification in sea urchin larvae are still not well understood. Primary mesenchyme cells within the larval body cavity form a syncytium to secrete CaCO3 spicules from intracellular amorphous CaCO3 (ACC) stores. We studied the role of Na+K+2Cl- cotransporter (NKCC) in int...
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ftdatacite:10.1594/pangaea.858067 2024-03-31T07:54:46+00:00 A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae ... Basse, Wiebke C Gutowska, Magdalena A Findeisen, Ulrike Stumpp, Meike Dupont, Sam Jackson, Daniel J Himmerkus, Nina Melzner, Frank Bleich, Markus 2016 application/zip https://dx.doi.org/10.1594/pangaea.858067 https://doi.pangaea.de/10.1594/PANGAEA.858067 en eng PANGAEA https://dx.doi.org/10.1016/j.cbpa.2015.05.005 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 Biological Impacts of Ocean Acidification BIOACID dataset Dataset Supplementary Dataset 2016 ftdatacite https://doi.org/10.1594/pangaea.85806710.1016/j.cbpa.2015.05.005 2024-03-04T12:56:15Z The cellular mechanisms of calcification in sea urchin larvae are still not well understood. Primary mesenchyme cells within the larval body cavity form a syncytium to secrete CaCO3 spicules from intracellular amorphous CaCO3 (ACC) stores. We studied the role of Na+K+2Cl- cotransporter (NKCC) in intracellular ACC accumulation and larval spicule formation of Strongylocentrotus droebachiensis. First, we incubated growing larvae with three different loop diuretics (azosemide, bumetanide, and furosemide) and established concentration-response curves. All loop diuretics were able to inhibit calcification already at concentrations that specifically inhibit NKCC. Calcification was most effectively inhibited by azosemide (IC50 = 6.5 µM), while larval mortality and swimming ability were not negatively impacted by the treatment. The inhibition by bumetanide (IC50 = 26.4 µM) and furosemide (IC50 = 315.4 µM) resembled the pharmacological fingerprint of the mammalian NKCC1 isoform. We further examined the effect of ... : Supplement to: Basse, Wiebke C; Gutowska, Magdalena A; Findeisen, Ulrike; Stumpp, Meike; Dupont, Sam; Jackson, Daniel J; Himmerkus, Nina; Melzner, Frank; Bleich, Markus (2015): A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 187, 184-192 ... Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Biological Impacts of Ocean Acidification BIOACID |
spellingShingle |
Biological Impacts of Ocean Acidification BIOACID Basse, Wiebke C Gutowska, Magdalena A Findeisen, Ulrike Stumpp, Meike Dupont, Sam Jackson, Daniel J Himmerkus, Nina Melzner, Frank Bleich, Markus A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae ... |
topic_facet |
Biological Impacts of Ocean Acidification BIOACID |
description |
The cellular mechanisms of calcification in sea urchin larvae are still not well understood. Primary mesenchyme cells within the larval body cavity form a syncytium to secrete CaCO3 spicules from intracellular amorphous CaCO3 (ACC) stores. We studied the role of Na+K+2Cl- cotransporter (NKCC) in intracellular ACC accumulation and larval spicule formation of Strongylocentrotus droebachiensis. First, we incubated growing larvae with three different loop diuretics (azosemide, bumetanide, and furosemide) and established concentration-response curves. All loop diuretics were able to inhibit calcification already at concentrations that specifically inhibit NKCC. Calcification was most effectively inhibited by azosemide (IC50 = 6.5 µM), while larval mortality and swimming ability were not negatively impacted by the treatment. The inhibition by bumetanide (IC50 = 26.4 µM) and furosemide (IC50 = 315.4 µM) resembled the pharmacological fingerprint of the mammalian NKCC1 isoform. We further examined the effect of ... : Supplement to: Basse, Wiebke C; Gutowska, Magdalena A; Findeisen, Ulrike; Stumpp, Meike; Dupont, Sam; Jackson, Daniel J; Himmerkus, Nina; Melzner, Frank; Bleich, Markus (2015): A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 187, 184-192 ... |
format |
Dataset |
author |
Basse, Wiebke C Gutowska, Magdalena A Findeisen, Ulrike Stumpp, Meike Dupont, Sam Jackson, Daniel J Himmerkus, Nina Melzner, Frank Bleich, Markus |
author_facet |
Basse, Wiebke C Gutowska, Magdalena A Findeisen, Ulrike Stumpp, Meike Dupont, Sam Jackson, Daniel J Himmerkus, Nina Melzner, Frank Bleich, Markus |
author_sort |
Basse, Wiebke C |
title |
A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae ... |
title_short |
A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae ... |
title_full |
A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae ... |
title_fullStr |
A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae ... |
title_full_unstemmed |
A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae ... |
title_sort |
sea urchin na+k+2cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in strongylocentrotus droebachiensis larvae ... |
publisher |
PANGAEA |
publishDate |
2016 |
url |
https://dx.doi.org/10.1594/pangaea.858067 https://doi.pangaea.de/10.1594/PANGAEA.858067 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://dx.doi.org/10.1016/j.cbpa.2015.05.005 |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_doi |
https://doi.org/10.1594/pangaea.85806710.1016/j.cbpa.2015.05.005 |
_version_ |
1795035966995431424 |