A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae ...

• Main Authors: Basse, Wiebke C, Gutowska, Magdalena A, Findeisen, Ulrike, Stumpp, Meike, Dupont, Sam, Jackson, Daniel J, Himmerkus, Nina, Melzner, Frank, Bleich, Markus
• Format: Dataset
• Language: English
• Published: PANGAEA 2016
• Subjects: Biological Impacts of Ocean Acidification BIOACID; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.858067; https://doi.pangaea.de/10.1594/PANGAEA.858067

The cellular mechanisms of calcification in sea urchin larvae are still not well understood. Primary mesenchyme cells within the larval body cavity form a syncytium to secrete CaCO3 spicules from intracellular amorphous CaCO3 (ACC) stores. We studied the role of Na+K+2Cl- cotransporter (NKCC) in intracellular ACC accumulation and larval spicule formation of Strongylocentrotus droebachiensis. First, we incubated growing larvae with three different loop diuretics (azosemide, bumetanide, and furosemide) and established concentration-response curves. All loop diuretics were able to inhibit calcification already at concentrations that specifically inhibit NKCC. Calcification was most effectively inhibited by azosemide (IC50 = 6.5 µM), while larval mortality and swimming ability were not negatively impacted by the treatment. The inhibition by bumetanide (IC50 = 26.4 µM) and furosemide (IC50 = 315.4 µM) resembled the pharmacological fingerprint of the mammalian NKCC1 isoform. We further examined the effect of ... : Supplement to: Basse, Wiebke C; Gutowska, Magdalena A; Findeisen, Ulrike; Stumpp, Meike; Dupont, Sam; Jackson, Daniel J; Himmerkus, Nina; Melzner, Frank; Bleich, Markus (2015): A sea urchin Na+K+2Cl- cotransporter is involved in the maintenance of calcification-relevant cytoplasmic cords in Strongylocentrotus droebachiensis larvae. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 187, 184-192 ...