Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) ...
Effects of severe hypercapnia have been extensively studied in marine fishes, while knowledge on the impacts of moderately elevated CO2 levels and their combination with warming is scarce. Here we investigate ion regulation mechanisms and energy budget in gills from Atlantic cod acclimated long-term...
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ftdatacite:10.1594/pangaea.848599 2024-03-31T07:51:28+00:00 Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) ... Kreiss, Cornelia M Michael, Katharina Bock, Christian Lucassen, Magnus Pörtner, Hans-Otto 2015 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.848599 https://doi.pangaea.de/10.1594/PANGAEA.848599 en eng PANGAEA https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1016/j.cbpa.2014.12.019 https://cran.r-project.org/package=seacarb Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 Animalia Chordata Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Gadus morhua Laboratory experiment Nekton North Atlantic Pelagos Respiration Single species Temperate Temperature Species Figure Tissues Concentration Partial pressure of carbon dioxide water at sea surface temperature wet air Treatment temperature Respiration rate, oxygen Respiration rate, oxygen, standard deviation Description Fraction Standard deviation Enzyme Adenosine triphosphatase activity Sodium/Potassium adenosine triphosphatase activity Temperature, water Temperature, water, standard deviation Partial pressure of carbon dioxide, standard deviation pH pH, standard deviation Salinity Salinity, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Calculated CTD, Sea-Bird SBE 911plus Calculated using seacarb after Nisumaa et al. 2010 Biological Impacts of Ocean Acidification BIOACID Ocean Acidification International Coordination Centre OA-ICC dataset Dataset Supplementary Dataset 2015 ftdatacite https://doi.org/10.1594/pangaea.84859910.1016/j.cbpa.2014.12.019 2024-03-04T14:19:23Z Effects of severe hypercapnia have been extensively studied in marine fishes, while knowledge on the impacts of moderately elevated CO2 levels and their combination with warming is scarce. Here we investigate ion regulation mechanisms and energy budget in gills from Atlantic cod acclimated long-term to elevated PCO2 levels (2500 µatm) and temperature (18 °C). Isolated perfused gill preparations established to determine gill thermal plasticity during acute exposures (10-22 °C) and in vivo costs of Na+/K+-ATPase activity, protein and RNA synthesis. Maximum enzyme capacities of F1Fo-ATPase, H+-ATPase and Na+/K+-ATPase were measured in vitro in crude gill homogenates. After whole animal acclimation to elevated PCO2 and/or warming, branchial oxygen consumption responded more strongly to acute temperature change. The fractions of gill respiration allocated to protein and RNA synthesis remained unchanged. In gills of fish CO2-exposed at both temperatures, energy turnover associated with Na+/K+-ATPase activity was ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-08-06 ... Dataset atlantic cod Gadus morhua North Atlantic Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Animalia Chordata Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Gadus morhua Laboratory experiment Nekton North Atlantic Pelagos Respiration Single species Temperate Temperature Species Figure Tissues Concentration Partial pressure of carbon dioxide water at sea surface temperature wet air Treatment temperature Respiration rate, oxygen Respiration rate, oxygen, standard deviation Description Fraction Standard deviation Enzyme Adenosine triphosphatase activity Sodium/Potassium adenosine triphosphatase activity Temperature, water Temperature, water, standard deviation Partial pressure of carbon dioxide, standard deviation pH pH, standard deviation Salinity Salinity, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Calculated CTD, Sea-Bird SBE 911plus Calculated using seacarb after Nisumaa et al. 2010 Biological Impacts of Ocean Acidification BIOACID Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Animalia Chordata Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Gadus morhua Laboratory experiment Nekton North Atlantic Pelagos Respiration Single species Temperate Temperature Species Figure Tissues Concentration Partial pressure of carbon dioxide water at sea surface temperature wet air Treatment temperature Respiration rate, oxygen Respiration rate, oxygen, standard deviation Description Fraction Standard deviation Enzyme Adenosine triphosphatase activity Sodium/Potassium adenosine triphosphatase activity Temperature, water Temperature, water, standard deviation Partial pressure of carbon dioxide, standard deviation pH pH, standard deviation Salinity Salinity, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Calculated CTD, Sea-Bird SBE 911plus Calculated using seacarb after Nisumaa et al. 2010 Biological Impacts of Ocean Acidification BIOACID Ocean Acidification International Coordination Centre OA-ICC Kreiss, Cornelia M Michael, Katharina Bock, Christian Lucassen, Magnus Pörtner, Hans-Otto Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) ... |
topic_facet |
Animalia Chordata Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Gadus morhua Laboratory experiment Nekton North Atlantic Pelagos Respiration Single species Temperate Temperature Species Figure Tissues Concentration Partial pressure of carbon dioxide water at sea surface temperature wet air Treatment temperature Respiration rate, oxygen Respiration rate, oxygen, standard deviation Description Fraction Standard deviation Enzyme Adenosine triphosphatase activity Sodium/Potassium adenosine triphosphatase activity Temperature, water Temperature, water, standard deviation Partial pressure of carbon dioxide, standard deviation pH pH, standard deviation Salinity Salinity, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Calculated CTD, Sea-Bird SBE 911plus Calculated using seacarb after Nisumaa et al. 2010 Biological Impacts of Ocean Acidification BIOACID Ocean Acidification International Coordination Centre OA-ICC |
description |
Effects of severe hypercapnia have been extensively studied in marine fishes, while knowledge on the impacts of moderately elevated CO2 levels and their combination with warming is scarce. Here we investigate ion regulation mechanisms and energy budget in gills from Atlantic cod acclimated long-term to elevated PCO2 levels (2500 µatm) and temperature (18 °C). Isolated perfused gill preparations established to determine gill thermal plasticity during acute exposures (10-22 °C) and in vivo costs of Na+/K+-ATPase activity, protein and RNA synthesis. Maximum enzyme capacities of F1Fo-ATPase, H+-ATPase and Na+/K+-ATPase were measured in vitro in crude gill homogenates. After whole animal acclimation to elevated PCO2 and/or warming, branchial oxygen consumption responded more strongly to acute temperature change. The fractions of gill respiration allocated to protein and RNA synthesis remained unchanged. In gills of fish CO2-exposed at both temperatures, energy turnover associated with Na+/K+-ATPase activity was ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-08-06 ... |
format |
Dataset |
author |
Kreiss, Cornelia M Michael, Katharina Bock, Christian Lucassen, Magnus Pörtner, Hans-Otto |
author_facet |
Kreiss, Cornelia M Michael, Katharina Bock, Christian Lucassen, Magnus Pörtner, Hans-Otto |
author_sort |
Kreiss, Cornelia M |
title |
Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) ... |
title_short |
Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) ... |
title_full |
Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) ... |
title_fullStr |
Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) ... |
title_full_unstemmed |
Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) ... |
title_sort |
impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of atlantic cod (gadus morhua) ... |
publisher |
PANGAEA |
publishDate |
2015 |
url |
https://dx.doi.org/10.1594/pangaea.848599 https://doi.pangaea.de/10.1594/PANGAEA.848599 |
genre |
atlantic cod Gadus morhua North Atlantic Ocean acidification |
genre_facet |
atlantic cod Gadus morhua North Atlantic Ocean acidification |
op_relation |
https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1016/j.cbpa.2014.12.019 https://cran.r-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_doi |
https://doi.org/10.1594/pangaea.84859910.1016/j.cbpa.2014.12.019 |
_version_ |
1795030100481146880 |