Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) ...

• Main Authors: Kreiss, Cornelia M, Michael, Katharina, Bock, Christian, Lucassen, Magnus, Pörtner, Hans-Otto
• Format: Dataset
• Language: English
• Published: PANGAEA 2015
• Subjects: Animalia; Chordata; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Gadus morhua; Laboratory experiment; Nekton; North Atlantic; Pelagos; Respiration; Single species; Temperate; Temperature; Species; Figure; Tissues; Concentration; Partial pressure of carbon dioxide water at sea surface temperature wet air; Treatment temperature; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Description; Fraction; Standard deviation; Enzyme; Adenosine triphosphatase activity; Sodium/Potassium adenosine triphosphatase activity; Temperature, water; Temperature, water, standard deviation; Partial pressure of carbon dioxide, standard deviation; pH; pH, standard deviation; Salinity; Salinity, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Alkalinity, total; Aragonite saturation state; Calcite saturation state; Calculated; CTD, Sea-Bird SBE 911plus; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; Ocean Acidification International Coordination Centre OA-ICC; atlantic cod; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.848599; https://doi.pangaea.de/10.1594/PANGAEA.848599

Effects of severe hypercapnia have been extensively studied in marine fishes, while knowledge on the impacts of moderately elevated CO2 levels and their combination with warming is scarce. Here we investigate ion regulation mechanisms and energy budget in gills from Atlantic cod acclimated long-term to elevated PCO2 levels (2500 µatm) and temperature (18 °C). Isolated perfused gill preparations established to determine gill thermal plasticity during acute exposures (10-22 °C) and in vivo costs of Na+/K+-ATPase activity, protein and RNA synthesis. Maximum enzyme capacities of F1Fo-ATPase, H+-ATPase and Na+/K+-ATPase were measured in vitro in crude gill homogenates. After whole animal acclimation to elevated PCO2 and/or warming, branchial oxygen consumption responded more strongly to acute temperature change. The fractions of gill respiration allocated to protein and RNA synthesis remained unchanged. In gills of fish CO2-exposed at both temperatures, energy turnover associated with Na+/K+-ATPase activity was ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-08-06 ...