Adenylate concentration and energy charge in different thallus regions and after UV radiation in brown, red and green algae ...
A method was developed to extract adenine nucleotides AMP, ADP, and ATP from marine macroalgal tissue to gain information on the cellular energy charge. Quantification was carried out by high performance liquid chromatography (HPLC). Three species from the rocky shore of the island of Helgoland (Ger...
| Main Authors: | , , |
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| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
PANGAEA
2005
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.1594/pangaea.848162 https://doi.pangaea.de/10.1594/PANGAEA.848162 |
| Summary: | A method was developed to extract adenine nucleotides AMP, ADP, and ATP from marine macroalgal tissue to gain information on the cellular energy charge. Quantification was carried out by high performance liquid chromatography (HPLC). Three species from the rocky shore of the island of Helgoland (German Bight) were examined: Laminaria saccharina (Phaeophyta), Chondrus crispus (Rhodophyta), and Ulva lactuca (Chlorophyta). In L. saccharina and C. crispus, the adenylate energy charge (AEC) was determined in different thallus regions. AEC varied in relation to tissue age and function. Higher AEC values typically occurred in thallus regions with meristematic activity. Furthermore, L. saccharina and U. lactuca were exposed to UV-A and elevated UV-B radiation. The AEC was calculated and the maximal quantum yield of photosystem II (Fv/Fm) was determined as indicators for UV stress. In both species, the AEC remained at high values (0.72 ± 0.04), while Fv/Fm dropped rapidly. The results show that the photosynthesis of ... : Supplement to: Hünken, Mark; Karsten, Ulf; Wiencke, Christian (2005): Determination of the adenylate energy charge (AEC) as a tool to determine the physiological status of macroalgal tissues after UV exposure. Phycologia, 44(3), 249-253 ... |
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