The potential of ocean acidifi cation on suppressing larval development in the Pacifi c oyster Crassostrea gigas and blood cockle Arca infl ata Reeve, supplement to: Li, Jiaqi; Jiang, Zengjie; Zhang, Jihong; Mao, Yuze; Bian, Dapeng; Fang, Jianguang (2014): The potential of ocean acidification on suppressing larval development in the Pacific oyster Crassostrea gigas and blood cockle Arca inflata Reeve. Chinese Journal of Oceanology and Limnology, 32(6), 1307-1313

• Main Authors: Li, Jiaqi, Jiang, Zengjie, Zhang, Jihong, Mao, Yuze, Bian, Dapeng, Fang, Jianguang
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Animalia; Arca inflata Reeve; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Crassostrea gigas; Growth/Morphology; Laboratory experiment; Mollusca; North Pacific; Pelagos; Single species; Temperate; Zooplankton; Species; pH; Shell length; Shell length, standard deviation; Temperature, water; Alkalinity, total; Salinity; Carbonate system computation flag; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Potentiometric titration; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Fang; Pacific; Ocean acidification; Pacific oyster
• Online Access: https://dx.doi.org/10.1594/pangaea.841041; https://doi.pangaea.de/10.1594/PANGAEA.841041

We evaluated the effect of pH on larval development in larval Pacific oyster (Crassostrea gigas) and blood cockle ( Arca inflata Reeve). The larvae were reared at pH 8.2 (control), 7.9, 7.6, or 7.3 beginning 30 min or 24 h post fertilization. Exposure to lower pH during early embryonic development inhibited larval shell formation in both species. Compared with the control, larvae took longer to reach the D-veliger stage when reared under pH 7.6 and 7.3. Exposure to lower pH immediately after fertilization resulted in significantly delayed shell formation in the Pacific oyster larvae at pH 7.3 and blood cockle larvae at pH 7.6 and 7.3. However, when exposure was delayed until 24 h post fertilization, shell formation was only inhibited in blood cockle larvae reared at pH 7.3. Thus, the early embryonic stages were more sensitive to acidified conditions. Our results suggest that ocean acidification will have an adverse effect on embryonic development in bivalves. Although the effects appear subtle, they may accumulate and lead to subsequent issues during later larval development. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-12-25.