Impact of elevated pCO2 on paralytic shellfish poisoning toxin content and composition in Alexandrium tamarense ...

• Main Authors: Van de Waal, Dedmer B, Eberlein, Tim, John, Uwe, Wohlrab, Sylke, Rost, Björn
• Format: Dataset
• Language: English
• Published: PANGAEA 2014
• Subjects: Alexandrium tamarense; Bottles or small containers/Aquaria <20 L; Chromista; Gene expression incl. proteomics; Growth/Morphology; Immunology/Self-protection; Laboratory experiment; Laboratory strains; Myzozoa; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Species; Strain; Figure; Table; Partial pressure of carbon dioxide water at sea surface temperature wet air; Cellular paralytic shellfish toxin, total; Cellular paralytic shellfish toxin, total, standard deviation; Toxicity, cellular; Toxicity, cellular, standard deviation; Neurotoxin saxitoxin; Neurotoxin saxitoxin, standard deviation; Neosaxitoxin; Neosaxitoxin, standard deviation; Gonyautoxins 2/3; Gonyautoxins 2/3, standard deviation; Gonyautoxins 1/4; Gonyautoxins 1/4, standard deviation; Di-sulfated toxins C1+C2; Di-sulfated toxins C1+C2, standard deviation; Category; Gene abundance; Time in days; Cell density; Growth rate; Growth rate, standard deviation; Carbon, organic, particulate, production per cell; Particulate organic carbon, production, standard deviation; Carbon, organic, particulate, per cell; Carbon, organic, particulate, standard deviation; Nitrogen, organic, particulate, per cell; Nitrogen, organic, particulate, standard deviation; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Temperature, water; Salinity; Phosphate; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.835715; https://doi.pangaea.de/10.1594/PANGAEA.835715

Ocean acidification is considered a major threat to marine ecosystems and may particularly affect primary producers. Here we investigated the impact of elevated pCO2 on paralytic shellfish poisoning toxin (PST) content and composition in two strains of Alexandrium tamarense, Alex5 and Alex2. Experiments were carried out as dilute batch to keep carbonate chemistry unaltered over time. We observed only minor changes with respect to growth and elemental composition in response to elevated pCO2. For both strains, the cellular PST content, and in particular the associated cellular toxicity, was lower in the high CO2 treatments. In addition, Alex5 showed a shift in its PST composition from a nonsulfated analogue towards less toxic sulfated analogues with increasing pCO2. Transcriptomic analyses suggest that the ability of A. tamarense to maintain cellular homeostasis is predominantly regulated on the post-translational level rather than on the transcriptomic level. Furthermore, genes associated to secondary ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-11. ...