Impact of elevated pCO2 on paralytic shellfish poisoning toxin content and composition in Alexandrium tamarense, supplement to: Van de Waal, Dedmer B; Eberlein, Tim; John, Uwe; Wohlrab, Sylke; Rost, Bjoern (2014): Impact of elevated pCO2 on paralytic shellfish poisoning toxin content and composition in Alexandrium tamarense. Toxicon, 78, 58-67

• Main Authors: Van de Waal, Dedmer B, Eberlein, Tim, John, Uwe, Wohlrab, Sylke, Rost, Bjoern
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Alexandrium tamarense; Bottles or small containers/Aquaria <20 L; Chromista; Gene expression incl. proteomics; Growth/Morphology; Immunology/Self-protection; Laboratory experiment; Laboratory strains; Myzozoa; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Species; Strain; Figure; Table; Partial pressure of carbon dioxide water at sea surface temperature wet air; Cellular paralytic shellfish toxin, total; Cellular paralytic shellfish toxin, total, standard deviation; Toxicity, cellular; Toxicity, cellular, standard deviation; Neurotoxin saxitoxin; Neurotoxin saxitoxin, standard deviation; Neosaxitoxin; Neosaxitoxin, standard deviation; Gonyautoxins 2/3; Gonyautoxins 2/3, standard deviation; Gonyautoxins 1/4; Gonyautoxins 1/4, standard deviation; Di-sulfated toxins C1+C2; Di-sulfated toxins C1+C2, standard deviation; Category; Gene abundance; Time in days; Cell density; Growth rate; Growth rate, standard deviation; Production of particulate organic carbon per cell; Particulate organic carbon, production, standard deviation; Carbon, organic, particulate, per cell; Carbon, organic, particulate, standard deviation; Nitrogen, organic, particulate, per cell; Nitrogen, organic, particulate, standard deviation; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Temperature, water; Salinity; Phosphate; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Potentiometric; Potentiometric titration; Coulometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.835715; https://doi.pangaea.de/10.1594/PANGAEA.835715

Ocean acidification is considered a major threat to marine ecosystems and may particularly affect primary producers. Here we investigated the impact of elevated pCO2 on paralytic shellfish poisoning toxin (PST) content and composition in two strains of Alexandrium tamarense, Alex5 and Alex2. Experiments were carried out as dilute batch to keep carbonate chemistry unaltered over time. We observed only minor changes with respect to growth and elemental composition in response to elevated pCO2. For both strains, the cellular PST content, and in particular the associated cellular toxicity, was lower in the high CO2 treatments. In addition, Alex5 showed a shift in its PST composition from a nonsulfated analogue towards less toxic sulfated analogues with increasing pCO2. Transcriptomic analyses suggest that the ability of A. tamarense to maintain cellular homeostasis is predominantly regulated on the post-translational level rather than on the transcriptomic level. Furthermore, genes associated to secondary metabolite and amino acid metabolism in Alex5 were down-regulated in the high CO2 treatment, which may explain the lower PST content. Elevated pCO2 also induced up-regulation of a putative sulfotransferase sxtN homologue and a substantial down-regulation of several sulfatases. Such changes in sulfur metabolism may explain the shift in PST composition towards more sulfated analogues. All in all, our results indicate that elevated pCO2 will have minor consequences for growth and elemental composition, but may potentially reduce the cellular toxicity of A. tamarense. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-11.