Colimitation of the unicellular photosynthetic diazotroph Crocosphaera watsonii by phosphorus, light, and carbon dioxide ...

• Main Authors: Garcia, Nathan S, Fu, Feixue, Hutchins, David A
• Format: Dataset
• Language: English
• Published: PANGAEA 2013
• Subjects: Bacteria; Bottles or small containers/Aquaria <20 L; Crocosphaera watsonii; Cyanobacteria; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Macro-nutrients; North Pacific; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Species; Table; Figure; Time point, descriptive; Partial pressure of carbon dioxide water at sea surface temperature wet air; Irradiance; log-phosphorus, total; Growth rate; Phosphorus, total; Photosynthetic carbon fixation per cell, maximum velocity; Nitrogen fixation rate, per cell; Diameter; Diameter, standard error; Nitrogen fixation rate, gross, per cellular phosphorus; Carbon fixation rate, per cellular phosphorus; Photosynthetic carbon fixation rate per cell; Phosphorus uptake rate, per cell; Phosphorus uptake rate/growth rate ratio; Phosphorus, total, per cell; Cellular phosphorus, per cell volume; pH; Carbon, inorganic, dissolved; Temperature, water; Salinity; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbonate ion; Bicarbonate ion; Alkalinity, total; Aragonite saturation state; Calcite saturation state; Potentiometric; Coulometric titration; Calculated using CO2SYS; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.835394; https://doi.pangaea.de/10.1594/PANGAEA.835394

We describe interactive effects of total phosphorus (total P = 0.1-4.0 µmol/L; added as H2NaPO4), irradiance (40 and 150 µmol quanta/m**2/s), and the partial pressure of carbon dioxide (P-CO2; 19 and 81 Pa, i.e., 190 and 800 ppm) on growth and CO2- and dinitrogen (N-2)-fixation rates of the unicellular N-2-fixing cyanobacterium Crocosphaera watsonii (WH0003) isolated from the Pacific Ocean near Hawaii. In semicontinuous cultures of C. watsonii, elevated P-CO2 positively affected growth and CO2- and N-2-fixation rates under high light. Under low light, elevated P-CO2 positively affected growth rates at all concentrations of P, but CO2- and N-2-fixation rates were affected by elevated P-CO2 only when P was low. In both high-light and low-light cultures, the total P requirements for growth and CO2- and N-2-fixation declined as P-CO2 increased. The minimum concentration (C-min) of total P and half-saturation constant (K-1/2) for growth and CO2- and N-2-fixation rates with respect to total P were reduced by 0.05 ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-01. ...