Seawater acidification by CO2 in a coastal lagoon environment: Effects on life history traits of juvenile mussels Mytilus galloprovincialis, supplement to: Range, P; Pilò, D; Ben-Hamadou, R; ChÌcharo, M A; Matias, D; Joaquim, S; Oliveira, A P; ChÌcharo, L (2012): Seawater acidification by CO2 in a coastal lagoon environment: Effects on life history traits of juvenile mussels Mytilus galloprovincialis. Journal of Experimental Marine Biology and Ecology, 424-425, 89-98

• Main Authors: Range, P, Pilò, D, Ben-Hamadou, R, ChÌcharo, M A, Matias, D, Joaquim, S, Oliveira, A P, ChÌcharo, L
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2012
• Subjects: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus galloprovincialis; North Atlantic; Single species; Temperate; Species; Table; Figure; Treatment; Incubation duration; Growth rate; Size; Mass; Mass, standard error; Temperature, water; Temperature, water, standard error; Salinity; Salinity, standard error; pH; pH, standard error; Alkalinity, total; Alkalinity, total, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Calcite saturation state; Calcite saturation state, standard error; Aragonite saturation state; Aragonite saturation state, standard error; Phosphate; Phosphate, standard error; Silicate; Silicate, standard error; Condition index; Condition index, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Experiment; Potentiometric; Potentiometric titration; Calculated; Spectrophotometric; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.833632; https://doi.pangaea.de/10.1594/PANGAEA.833632

The carbonate chemistry of seawater from the Ria Formosa lagoon was experimentally manipulated, by diffusing pure CO2, to attain two reduced pH levels, by -0.3 and -0.6 pH units, relative to unmanipulated seawater. After 84 days of exposure, no differences were detected in terms of growth (somatic or shell) or mortality of juvenile mussels Mytilus galloprovincialis. The naturally elevated total alkalinity of the seawater (= 3550 µmol/kg) prevented under-saturation of CaCO3, even under pCO2 values exceeding 4000 µatm, attenuating the detrimental effects on the carbonate supply-side. Even so, variations in shell weight showed that net calcification was reduced under elevated CO2 and reduced pH, although the magnitude and significance of this effect varied among size-classes. Most of the loss of shell material probably occurred as post-deposition dissolution in the internal aragonitic nacre layer. Our results show that, even when reared under extreme levels of CO2-induced acidification, juvenile M. galloprovincialis can continue to calcify and grow in this coastal lagoon environment. The complex responses of bivalves to ocean acidification suggest a large degree of interspecific and intraspecific variability in their sensitivity to this type of perturbation. Further research is needed to assess the generality of these patterns and to disentangle the relative contributions of acclimation to local variations in seawater chemistry and genetic adaptation. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-06-26.