Simultaneous high-resolution pH and spectrophotometric recordings of oxygen binding in native blood microvolumes, link to supplementary material ...
Oxygen equilibrium curves have been widely used to understand oxygen transport in numerous organisms. A major challenge has been to monitor oxygen binding characteristics and concomitant pH changes as they occur in vivo, in limited sample volumes. Here we report a technique allowing highly resolved...
| Main Authors: | , , |
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| Format: | Dataset |
| Language: | English |
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PANGAEA
2014
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.1594/pangaea.831205 https://doi.pangaea.de/10.1594/PANGAEA.831205 |
| Summary: | Oxygen equilibrium curves have been widely used to understand oxygen transport in numerous organisms. A major challenge has been to monitor oxygen binding characteristics and concomitant pH changes as they occur in vivo, in limited sample volumes. Here we report a technique allowing highly resolved and simultaneous monitoring of pH and blood pigment saturation in minute blood volumes. We equipped a gas diffusion chamber with a broad range fibre optic spectrophotometer and a micro-pH optode and recorded changes of pigment oxygenation along PO2 and pH gradients to test the setup. Oxygen binding parameters derived from measurements in only 15 µl of haemolymph from the cephalopod Octopus vulgaris showed low instrumental error (0.93%) and good agreement with published data. Broad range spectra, each resolving 2048 data points, provided detailed insight into the complex absorbance characteristics of diverse blood types. After consideration of photobleaching and intrinsic fluorescence, pH optodes yielded accurate ... : Supplement to: Oellermann, Michael; Pörtner, Hans-Otto; Mark, Felix Christopher (2014): Simultaneous high-resolution pH and spectrophotometric recordings of oxygen binding in blood microvolumes. Journal of Experimental Biology, 217(9), 1430-1436 ... |
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