Experiment: Elevated temperature and PCO2 affect enzyme activities in differentially oxidative tissues of Notothenia rossii ...

Mitochondrial plasticity plays a central role in setting the capacity for acclimation of aerobic metabolism in ectotherms in response to environmental changes. We still lack a clear picture if and to what extent the energy metabolism and mitochondrial enzymes of Antarctic fish can compensate for cha...

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Bibliographic Details
Main Authors: Strobel, Anneli, Leo, Elettra, Pörtner, Hans-Otto, Mark, Felix Christopher
Format: Dataset
Language:English
Published: PANGAEA 2014
Subjects:
Identification
Species
Temperature, technical
Carbon dioxide, partial pressure
Citrate synthase activity, per protein
Cytochrome c oxidase activity, per protein
Citrate synthase activity per fresh mass
Cytochrome c oxidase activity, per fresh mass
Multiple investigations
Jubany_Dallmann
Carlini/Jubany Station
Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158
Antarctic
Arctic
The Antarctic
Antarc*
Notothenia rossii
Sea ice
Online Access:https://dx.doi.org/10.1594/pangaea.829831
https://doi.pangaea.de/10.1594/PANGAEA.829831
Description
Summary:Mitochondrial plasticity plays a central role in setting the capacity for acclimation of aerobic metabolism in ectotherms in response to environmental changes. We still lack a clear picture if and to what extent the energy metabolism and mitochondrial enzymes of Antarctic fish can compensate for changing temperatures or PCO2 and whether capacities for compensation differ between tissues. We therefore measured activities of key mitochondrial enzymes (citrate synthase (CS), cytochrome c oxidase (COX)) from heart, red muscle, white muscle and liver in the Antarctic fish Notothenia rossii after warm- (7 °C) and hypercapnia- (0.2 kPa CO2) acclimation vs. control conditions (1 °C, 0.04 kPa CO2). In heart, enzymes showed elevated activities after cold-hypercapnia acclimation, and a warm-acclimation-induced upward shift in thermal optima. The strongest increase in enzyme activities in response to hypercapnia occurred in red muscle. In white muscle, enzyme activities were temperature-compensated. CS activity in liver ... : Notothenia rossii acclimated to higher temperature and PCO2; Citrate synthase (CS) and cytochrome c oxidase (COX) activities are given in nmol per minute and mg protein (CS/protein; COX/protein) or mg tissue fresh mass (CS/MW; COX/MW) at the assay temperatures of 0, 6, 9, 12 °C in heart, liver, red muscle & white muscle tissue of all acclimated animals and the control. ...

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