Experiment: Elevated temperature and PCO2 affect enzyme activities in differentially oxidative tissues of Notothenia rossii ...
Mitochondrial plasticity plays a central role in setting the capacity for acclimation of aerobic metabolism in ectotherms in response to environmental changes. We still lack a clear picture if and to what extent the energy metabolism and mitochondrial enzymes of Antarctic fish can compensate for cha...
Main Authors: | , , , |
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Format: | Dataset |
Language: | English |
Published: |
PANGAEA
2014
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Subjects: | |
Online Access: | https://dx.doi.org/10.1594/pangaea.829831 https://doi.pangaea.de/10.1594/PANGAEA.829831 |
Summary: | Mitochondrial plasticity plays a central role in setting the capacity for acclimation of aerobic metabolism in ectotherms in response to environmental changes. We still lack a clear picture if and to what extent the energy metabolism and mitochondrial enzymes of Antarctic fish can compensate for changing temperatures or PCO2 and whether capacities for compensation differ between tissues. We therefore measured activities of key mitochondrial enzymes (citrate synthase (CS), cytochrome c oxidase (COX)) from heart, red muscle, white muscle and liver in the Antarctic fish Notothenia rossii after warm- (7 °C) and hypercapnia- (0.2 kPa CO2) acclimation vs. control conditions (1 °C, 0.04 kPa CO2). In heart, enzymes showed elevated activities after cold-hypercapnia acclimation, and a warm-acclimation-induced upward shift in thermal optima. The strongest increase in enzyme activities in response to hypercapnia occurred in red muscle. In white muscle, enzyme activities were temperature-compensated. CS activity in liver ... : Notothenia rossii acclimated to higher temperature and PCO2; Citrate synthase (CS) and cytochrome c oxidase (COX) activities are given in nmol per minute and mg protein (CS/protein; COX/protein) or mg tissue fresh mass (CS/MW; COX/MW) at the assay temperatures of 0, 6, 9, 12 °C in heart, liver, red muscle & white muscle tissue of all acclimated animals and the control. ... |
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