The morphological response of Emiliania huxleyi to seawater carbonate chemistry changes: an inter-strain comparison ...

• Main Authors: Langer, Gerald, Probert, Ian, Nehrke, Gernot, Ziveri, Patrizia
• Format: Dataset
• Language: English
• Published: PANGAEA 2011
• Subjects: Bottles or small containers/Aquaria <20 L; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species; Event label; Species; Strain; Replicates; Partial pressure of carbon dioxide water at sea surface temperature wet air; Coccoliths, normal; Coccoliths, normal, standard deviation; Malformation rate; Malformation rate, standard deviation; Coccoliths, incomplete; Coccoliths, incomplete, standard deviation; Coccoliths, malformed and incomplete; Coccoliths, malformed and incomplete, standard deviation; Nitrate; Phosphate; Alkalinity, total; Carbon, inorganic, dissolved; pH; Bicarbonate ion; Carbonate ion; Calcite saturation state; Salinity; Temperature, water; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Experiment; Calculated using CO2SYS; Potentiometric titration; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; European Project on Ocean Acidification EPOCA; Mediterranean Sea Acidification in a Changing Climate MedSeA; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.826456; https://doi.pangaea.de/10.1594/PANGAEA.826456

Four strains of the coccolithophore Emiliania huxleyi (RCC1212, RCC1216, RCC1238, RCC1256) were grown in dilute batch culture at four CO2 levels ranging from ~200 µatm to ~1200 µatm. Coccolith morphology was analyzed based on scanning electron micrographs. Three of the four strains did not exhibit a change in morphology over the CO2 range tested. One strain (RCC1256) displayed an increase in the percentage of malformed coccoliths with increasing CO2 concentration. We conclude that the sensitivity of the coccolith-shaping machinery to carbonate chemistry changes is strain-specific. Although it has been shown before that carbonate chemistry related changes in growth- and calcification rate are strain-specific, there seems to be no consistent correlation between coccolith morphology and growth or calcification rate. We did not observe an increase in the percentage of incomplete coccoliths in RCC1256, indicating that the coccolith-shaping machinery per se is affected by acidification and not the signalling ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-01-20. ...