Bacteria and cyanobacteria abundance measured on water bottle samples during BAFFIN cruise BAF89/3 ...

Bacterial countsSamples for enumeration of bacterial abundance were drawn from Niskin bottles and immediately preserved in 20 mL glass scintillation vials with 0.2 µm filtered (Millex-GS, Millipore Corp.) formalin (2% final concentration). Preserved samples were stored dark in a refrigerator. Each s...

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Bibliographic Details
Main Author: Li, William K W
Format: Dataset
Language:English
Published: PANGAEA 2013
Subjects:
Event label
Date/Time of event
Latitude of event
Longitude of event
DEPTH, water
Bacteria
Cyanobacteria
Bottle, Niskin
Counting
BAF89/3
Baffin
Joint Global Ocean Flux Study JGOFS
Online Access:https://dx.doi.org/10.1594/pangaea.808255
https://doi.pangaea.de/10.1594/PANGAEA.808255
Description
Summary:Bacterial countsSamples for enumeration of bacterial abundance were drawn from Niskin bottles and immediately preserved in 20 mL glass scintillation vials with 0.2 µm filtered (Millex-GS, Millipore Corp.) formalin (2% final concentration). Preserved samples were stored dark in a refrigerator. Each sample was poured over triplicate polycarbonate membranes (0.2 µm black Nuclepore) underlain by pre-wetted backing filters (Sartorius #11306) which aid in distributing cells in an even manner on the Nuclepore surface. Cells were stained for 10 minutes with DAPI at a final concentration of 2 µg/ml and then drawn through the filters at a vacuum pressure of about 150 mm Hg. The filters were examined by epifluorescence microscopy (Leitz Orthoplan) under ultraviolet excitation from a mercury arc lamp (HBO 100W).Cyanobacterial countsSamples for enumeration of cyanobacterial abundance (20-100 ml) were freshly filtered in triplicate onto 0.2 µm black Nuclepore membranes which were underlain by backing filters. The ...

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