Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012, supplement to: Nakamura, Masoko; Morita, Masaya; Kurihara, Haruko; Mitarai, Satoshi (2012): Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades. Biology Open, 1(2), 75-81

• Main Authors: Nakamura, Masoko, Morita, Masaya, Kurihara, Haruko, Mitarai, Satoshi
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2012
• Subjects: Acropora digitifera; Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Cnidaria; Coast and continental shelf; Gene expression incl. proteomics; Laboratory experiment; North Pacific; Single species; Temperate; Experimental treatment; Experiment day; Salinity; Temperature, water; pH; pH, standard error; Alkalinity, total; Alkalinity, total, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Bicarbonate ion; Bicarbonate ion, standard error; Carbonate ion; Carbonate ion, standard error; Aragonite saturation state; Aragonite saturation state, standard deviation; Acropora digitifera, heat shock protein 70 expression; Acropora digitifera, heat shock protein 70 expression, standard error; Acropora digitifera, heat shock protein 90 expression; Acropora digitifera, heat shock protein 90 expression, standard error; Acropora digitifera, heat shock factor 1 expression; Acropora digitifera, heat shock factor 1 expression, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbon, inorganic, dissolved; Calcite saturation state; Salinometer AS ONE, IS/Mill-E; pH meter Mettler Toledo, USA; Total alkalinity titration analyzer Kimoto ATT-05, Japan; Calculated using CO2SYS; Calculated; see references; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Toledo; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.779704; https://doi.pangaea.de/10.1594/PANGAEA.779704

Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsps and hsf1 by quantitative real-time polymerase chain reaction (PCR). Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO2conditions. These findings indicate that the expression of hsps is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).