qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions
The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from adult (entire reproductive season) Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for any...
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PANGAEA - Data Publisher for Earth & Environmental Science
2011
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Online Access: | https://dx.doi.org/10.1594/pangaea.772212 https://doi.pangaea.de/10.1594/PANGAEA.772212 |
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ftdatacite:10.1594/pangaea.772212 2023-05-15T15:31:25+02:00 qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions Anderson, Kelli Elizur, Abigail 2011 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.772212 https://doi.pangaea.de/10.1594/PANGAEA.772212 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://dx.doi.org/10.1594/pangaea.810131 https://dx.doi.org/10.1186/1756-0500-5-21 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Event label DATE/TIME Treatment temperature Life Cycle state qPCR counts, mean qPCR counts, standard error of mean qPCR counts, ratio qPCR counts, ratio, standard error of mean Experimental tank 4 cubic metre under artificial conditions Fluorescence-based quantitative real-time PCR qPCR Dataset dataset 2011 ftdatacite https://doi.org/10.1594/pangaea.772212 https://doi.org/10.1594/pangaea.810131 https://doi.org/10.1186/1756-0500-5-21 2022-02-09T13:21:31Z The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from adult (entire reproductive season) Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for anyone wanting to study the effects of climate change/elevated temperature on reproductive physiology of fish (and perhaphs other vertebrates). In addition, a target gene (vitellogenin) has normalised using an inappropriate and an 'ideal' reference gene to demonstrate the consequences of using an unstable reference gene for normalisation.For the adult experiment, maiden and repeat adult females were held at the Salmon Enterprises of Tasmania (SALTAS) Wayatinah Hatchery (Tasmania, Australia) at ambient temperature and photoperiod in either 200 (maidens) or 50 (repeats) m3 circular tanks at stocking densities of 12-18, and 24-36 kg m-3 for maidens and repeats, respectively, until transfered to the experimental tanks. Dataset Atlantic salmon DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Event label DATE/TIME Treatment temperature Life Cycle state qPCR counts, mean qPCR counts, standard error of mean qPCR counts, ratio qPCR counts, ratio, standard error of mean Experimental tank 4 cubic metre under artificial conditions Fluorescence-based quantitative real-time PCR qPCR |
spellingShingle |
Event label DATE/TIME Treatment temperature Life Cycle state qPCR counts, mean qPCR counts, standard error of mean qPCR counts, ratio qPCR counts, ratio, standard error of mean Experimental tank 4 cubic metre under artificial conditions Fluorescence-based quantitative real-time PCR qPCR Anderson, Kelli Elizur, Abigail qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
topic_facet |
Event label DATE/TIME Treatment temperature Life Cycle state qPCR counts, mean qPCR counts, standard error of mean qPCR counts, ratio qPCR counts, ratio, standard error of mean Experimental tank 4 cubic metre under artificial conditions Fluorescence-based quantitative real-time PCR qPCR |
description |
The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from adult (entire reproductive season) Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for anyone wanting to study the effects of climate change/elevated temperature on reproductive physiology of fish (and perhaphs other vertebrates). In addition, a target gene (vitellogenin) has normalised using an inappropriate and an 'ideal' reference gene to demonstrate the consequences of using an unstable reference gene for normalisation.For the adult experiment, maiden and repeat adult females were held at the Salmon Enterprises of Tasmania (SALTAS) Wayatinah Hatchery (Tasmania, Australia) at ambient temperature and photoperiod in either 200 (maidens) or 50 (repeats) m3 circular tanks at stocking densities of 12-18, and 24-36 kg m-3 for maidens and repeats, respectively, until transfered to the experimental tanks. |
format |
Dataset |
author |
Anderson, Kelli Elizur, Abigail |
author_facet |
Anderson, Kelli Elizur, Abigail |
author_sort |
Anderson, Kelli |
title |
qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
title_short |
qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
title_full |
qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
title_fullStr |
qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
title_full_unstemmed |
qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
title_sort |
qpcr counts of mrna for hepatic reference gene selection in adult female atlantic salmon from perturbation experiments under artificial temperature conditions |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2011 |
url |
https://dx.doi.org/10.1594/pangaea.772212 https://doi.pangaea.de/10.1594/PANGAEA.772212 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://dx.doi.org/10.1594/pangaea.810131 https://dx.doi.org/10.1186/1756-0500-5-21 |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.772212 https://doi.org/10.1594/pangaea.810131 https://doi.org/10.1186/1756-0500-5-21 |
_version_ |
1766361919672811520 |