qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions

The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from adult (entire reproductive season) Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for any...

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Bibliographic Details
Main Authors: Anderson, Kelli, Elizur, Abigail
Format: Dataset
Language:English
Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
Subjects:
Event label
DATE/TIME
Treatment temperature
Life Cycle state
qPCR counts, mean
qPCR counts, standard error of mean
qPCR counts, ratio
qPCR counts, ratio, standard error of mean
Experimental tank 4 cubic metre under artificial conditions
Fluorescence-based quantitative real-time PCR qPCR
Atlantic salmon
Online Access:https://dx.doi.org/10.1594/pangaea.772212
https://doi.pangaea.de/10.1594/PANGAEA.772212
Description
Summary:The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from adult (entire reproductive season) Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for anyone wanting to study the effects of climate change/elevated temperature on reproductive physiology of fish (and perhaphs other vertebrates). In addition, a target gene (vitellogenin) has normalised using an inappropriate and an 'ideal' reference gene to demonstrate the consequences of using an unstable reference gene for normalisation.For the adult experiment, maiden and repeat adult females were held at the Salmon Enterprises of Tasmania (SALTAS) Wayatinah Hatchery (Tasmania, Australia) at ambient temperature and photoperiod in either 200 (maidens) or 50 (repeats) m3 circular tanks at stocking densities of 12-18, and 24-36 kg m-3 for maidens and repeats, respectively, until transfered to the experimental tanks.

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