Seawater carbonate chemistry and energy status in the periwinkle Littorina littorea during experiments, 2011, supplement to: Melatunan, Sedercor; Calosi, Piero; Rundle, Simon; Moody, A John; Widdicombe, Stephen (2011): Exposure to elevated temperature and pCO2 reduces respiration rate and energy status in the periwinkle Littorina littorea. Physiological and Biochemical Zoology, 84(6), 583-594

• Main Authors: Melatunan, Sedercor, Calosi, Piero, Rundle, Simon, Moody, A John, Widdicombe, Stephen
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Litiorina littorea; Mollusca; North Atlantic; Respiration; Single species; Temperate; Temperature; Experimental treatment; Species; Oxygen; Oxygen, standard deviation; Salinity; Salinity, standard deviation; Temperature, water; Temperature, standard deviation; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbon dioxide, partial pressure, standard deviation; Bicarbonate ion; Aragonite saturation state, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Respiration rate, oxygen; Oxygen consumption, standard deviation; Littorina littorea, adenosine triphosphate concentration; Littorina littorea, adenosine triphosphate concentration, standard deviation; Littorina littorea, adenosine diphosphate concentration; Littorina littorea, adenosine diphosphate concentration, standard deviation; Littorina littorea, adenosine monophosphate concentration; Littorina littorea, adenosine monophosphate concentration, standard deviation; Adenylate energy charge ratio; Adenylate energy charge ratio, standard deviation; Littorina littorea, total adenylate nucleotides; Littorina littorea, total adenylate nucleotides, standard deviation; Littorina littorea, succinate; Littorina littorea, succinate, standard deviation; Littorina littorea, D-lactate; Littorina littorea, D-lactate, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; YSI Multiparameter system; pH meter Mettler Toledo, USA; Automated CO2 analyzer CIBA-Corning 965, UK; Calculated using CO2SYS; Calculated, see references; see references; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Toledo; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.771574; https://doi.pangaea.de/10.1594/PANGAEA.771574

In the future, marine organisms will face the challenge of coping with multiple environmental changes associated with increased levels of atmospheric Pco2, such as ocean warming and acidification. To predict how organisms may or may not meet these challenges, an in-depth understanding of the physiological and biochemical mechanisms underpinning organismal responses to climate change is needed. Here, we investigate the effects of elevated Pco2 and temperature on the whole-organism and cellular physiology of the periwinkle Littorina littorea. Metabolic rates (measured as respiration rates), adenylate energy nucleotide concentrations and indexes, and end-product metabolite concentrations were measured. Compared with values for control conditions, snails decreased their respiration rate by 31% in response to elevated Pco2 and by 15% in response to a combination of increased Pco2 and temperature. Decreased respiration rates were associated with metabolic reduction and an increase in end-product metabolites in acidified treatments, indicating an increased reliance on anaerobic metabolism. There was also an interactive effect of elevated Pco2 and temperature on total adenylate nucleotides, which was apparently compensated for by the maintenance of adenylate energy charge via AMP deaminase activity. Our findings suggest that marine intertidal organisms are likely to exhibit complex physiological responses to future environmental drivers, with likely negative effects on growth, population dynamics, and, ultimately, ecosystem processes. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).