Seawater carbonate chemistry and processes during experiments with cyanobacterium Trichodesmium (IMS101), 2009 ...

• Main Authors: Kranz, Sven A, Sültemeyer, Dieter, Richter, Klaus-Uwe, Rost, Björn
• Format: Dataset
• Language: English
• Published: PANGAEA 2009
• Subjects: Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Cyanobacteria; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species; Trichodesmium sp.; Experimental treatment; Salinity; Temperature, water; Radiation, photosynthetically active; Carbonate system computation flag; Phosphate; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon dioxide, total; Carbon dioxide, standard deviation; Carbon dioxide; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Growth rate; Growth rate, standard deviation; Particulate organic nitrogen per cell; Particulate organic nitrogen per cell, standard deviation; Particulate organic carbon, per cell; Particulate organic carbon content per cell, standard deviation; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Conductivity meter WTW, Weilheim, Gemany; Walz 4pi sensor; Calculated using seacarb after Nisumaa et al. 2010; pH, Electrode; Calculated using CO2SYS; Alkalinity, Gran titration Gran, 1950; Calculated; Based on changes in chla/cells/POC/PON; Mass spectrometer ANCA-SL 20-20 Europa Scientific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.736024; https://doi.pangaea.de/10.1594/PANGAEA.736024

We investigated carbon acquisition by the N2-fixing cyanobacterium Trichodesmium IMS101 in response to CO2 levels of 15.1, 37.5, and 101.3 Pa (equivalent to 150, 370, and 1000 ppm). In these acclimations, growth rates as well as cellular C and N contents were measured. In vivo activities of carbonic anhydrase (CA), photosynthetic O2 evolution, and CO2 and HCO3- fluxes were measured using membrane inlet mass spectrometry and the 14C disequilibrium technique. While no differences in growth rates were observed, elevated CO2 levels caused higher C and N quotas and stimulated photosynthesis and N2 fixation. Minimal extracellular CA (eCA) activity was observed, indicating a minor role in carbon acquisition. Rates of CO2 uptake were small relative to total inorganic carbon (Ci) fixation, whereas HCO{3 contributed more than 90% and varied only slightly over the light period and between CO2 treatments. The low eCA activity and preference for HCO3- were verified by the 14C disequilibrium technique. Regarding apparent ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). ...