Seawater carbonate chemistry and biological processes during experiments with four species of marine diatoms ...

• Main Authors: Trimborn, Scarlett, Wolf-Gladrow, Dieter A, Richter, Klaus-Uwe, Rost, Björn
• Format: Dataset
• Language: English
• Published: PANGAEA 2009
• Subjects: Bottles or small containers/Aquaria <20 L; Chromista; Eucampia zoodiacus; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Skeletonema costatum; Thalassionema nitzschioides; Thalassiosira pseudonana; Experimental treatment; Species; Salinity; Temperature, water; LightDark cycle; Radiation, photosynthetically active; pH; Alkalinity, total; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbon dioxide; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Extracellular carbonic anhydrase activity; Intracellular carbonic anhydrase activity per chlorophyll a; Bicarbonate uptake; Isotopic fractionation, during photosynthis; pH meter, WTW, pH 3000; Calculated using seacarb after Nisumaa et al. 2010; Alkalinity, Gran titration Gran, 1950; Calculated using CO2SYS; Measured by loss of 18O Silverman, 1982; Calculated; Calculated after Freeman & Hayes 1992; see references; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158; Arctic; Antarctic; Hayes; Heimdal; Antarc*; Ocean acidification; Sea ice
• Online Access: https://dx.doi.org/10.1594/pangaea.733948; https://doi.pangaea.de/10.1594/PANGAEA.733948

The effect of pCO2 on carbon acquisition and intracellular assimilation was investigated in the three bloom-forming diatom species, Eucampia zodiacus (Ehrenberg), Skeletonema costatum (Greville) Cleve, Thalassionema nitzschioides (Grunow) Mereschkowsky and the non-bloom-forming Thalassiosira pseudonana (Hust.) Hasle and Heimdal. In vivo activities of carbonic anhydrase (CA), photosynthetic O2 evolution, CO2 and HCO3? uptake rates were measured by membrane-inlet mass spectrometry (MIMS) in cells acclimated to pCO2 levels of 370 and 800 ?atm. To investigate whether the cells operate a C4-like pathway, activities of ribulose-1,5-bisphosphate carboxylase (RubisCO) and phosphoenolpyruvate carboxylase (PEPC) were measured at the mentioned pCO2 levels and a lower pCO2 level of 50 ?atm. In the bloom-forming species, extracellular CA activities strongly increased with decreasing CO2 supply while constantly low activities were obtained for T. pseudonana. Half-saturation concentrations (K1/2) for photosynthetic O2 ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). ...