Seawater carbonate chemistry and biological processes during experiments with four strains of Emiliania huxleyi ...

• Main Authors: Langer, Gerald, Nehrke, Gernot, Probert, Ian, Ly, J, Ziveri, Patrizia
• Format: Dataset
• Language: English
• Published: PANGAEA 2009
• Subjects: Bottles or small containers/Aquaria <20 L; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Event label; Identification; Carbonate system computation flag; Salinity; Temperature, water; Radiation, photosynthetically active; Nitrate; Phosphate; Alkalinity, total; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; pH; Bicarbonate ion; Carbonate ion; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Growth rate; Growth rate, standard deviation; Particulate inorganic carbon per cell; Particulate inorganic carbon per cell, standard deviation; Particulate organic carbon, per cell; Particulate organic carbon content per cell, standard deviation; Particulate inorganic carbon production per cell; Particulate inorganic carbon, production, standard deviation; Particulate organic carbon production per cell; Particulate organic carbon, production, standard deviation; Particulate inorganic carbon/particulate organic carbon ratio; Particulate inorganic carbon/particulate organic carbon ratio, standard deviation; Experiment; Conductivity meter WTW, Weilheim, Gemany; Alkalinity, Gran titration Gran, 1950; Photometry; Calculated; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Element analyser CNS, EURO EA; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.733946; https://doi.pangaea.de/10.1594/PANGAEA.733946

Four strains of the coccolithophore E. huxleyi (RCC1212, RCC1216, RCC1238, RCC1256) were grown in dilute batch culture at four CO2 levels ranging from ~200 µatm to ~1200 µatm. Growth rate, particulate organic carbon content, and particulate inorganic carbon content were measured, and organic and inorganic carbon production calculated. The four strains did not show a uniform response to carbonate chemistry changes in any of the analysed parameters and none of the four strains displayed a response pattern previously described for this species. We conclude that the sensitivity of different strains of E. huxleyi to acidification differs substantially and that this likely has a genetic basis. We propose that this can explain apparently contradictory results reported in the literature. ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). ...