Seawater carbonate chemistry during experiments with Mytilus edulis, 2008, supplement to: Bibby, Ruth; Widdicombe, Stephen; Parry, Helen E; Spicer, John I; Pipe, R (2008): Effects of ocean acidification on the immune response of the blue mussel Mytilus edulis. Aquatic Biology, 2(1), 97-74

• Main Authors: Bibby, Ruth, Widdicombe, Stephen, Parry, Helen E, Spicer, John I, Pipe, R
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2008
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Immunology/Self-protection; Laboratory experiment; Mollusca; Mytilus edulis; North Atlantic; Single species; Temperate; Experimental treatment; Salinity; Temperature, water; Carbonate system computation flag; pH; Alkalinity, total; Carbon, inorganic, dissolved; Carbon dioxide; Carbonate ion; Bicarbonate ion; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Phagocytosed particles, number per protein mass; Superoxyde dismutase change, number per protein mass; Blood cells; Basophil cells, absolute numbers; Eosinophil cells, absolute numbers; Basophil cells; Experiment; Tetra Con 325 salinity and temperature probe; Calculated using seacarb after Nisumaa et al. 2010; pH meter Mettler Toledo InLab 413 SG; SpectraMax microplate reader Molecular Devices; Neubauer haemocytometer; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Parry; Toledo; Bibby; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.718100; https://doi.pangaea.de/10.1594/PANGAEA.718100

The effects of medium term (32 d) hypercapnia on the immune response of Mytilus edulis were investigated in mussels exposed to acidified (using CO2) sea water (pH 7.7, 7.5 or 6.7; control: pH 7.8). Levels of phagocytosis increased significantly during the exposure period, suggesting an immune response induced by the experimental set-up. However, this induced stress response was suppressed when mussels were exposed to acidified sea water. Acidified sea water did not have any significant effects on other immuno-surveillance parameters measured (superoxide anion production, total and differential cell counts). These results suggest that ocean acidification may impact the physiological condition and functionality of the haemocytes and could have a significant effect on cellular signalling pathways, particularly those pathways that rely on specific concentrations of calcium, and so may be disrupted by calcium carbonate shell dissolution. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).