Quantitative PCR (qPCR) of Key Macroalgal Non-Indigenous Species in Nova Scotia and New Brunswick Waters ...

To support the surveillance of key macroalgae and non-indigenous species in Nova Scotia and New Brunswick, five quantitative PCR (qPCR) assays were designed and tested at 111 sites in 2022-2023 targeting the following non-indigenous macroalgal species: Antithamnion sparsum, Bonnemaisonia hamifera, C...

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Bibliographic Details
Main Authors: Krumhansl, Kira, DiBacco, Claudio
Format: Dataset
Language:English
Published: Fisheries and Oceans Canada 2024
Subjects:
samplingEvent
Occurrence
Oceans
invasive species
Dasysiphonia japonica
Bonnemaisonia hamifera
Macroalgae
qPCR
Codium fragile
Antithamnion sparsum
eDNA
non-indigenous species
Bedford
Canada
Newfoundland
Online Access:https://dx.doi.org/10.15468/kyhydn
https://www.gbif.org/dataset/8820bfe8-95de-4b9e-b323-9324d55deaf9
Description
Summary:To support the surveillance of key macroalgae and non-indigenous species in Nova Scotia and New Brunswick, five quantitative PCR (qPCR) assays were designed and tested at 111 sites in 2022-2023 targeting the following non-indigenous macroalgal species: Antithamnion sparsum, Bonnemaisonia hamifera, Codium fragile, Dasysiphonia japonica, Fucus serratus. All assays were developed in 2022 by the Center for Environmental Genomics Applications (CEGA, Newfoundland, Canada) except Antithamnion sparsum, for which an assay was developed in 2023 by the Aquatic Biotechnology Laboratory (ABL) at the Bedford Institute of Oceanography. All amplification was performed by the ABL in 2022-2023. The assay developed for Fucus serratus was later determined to be non-specific, and amplifies both F. serratus and Fucus distichus. ...

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