Endocytosis and membrane lipid composition in wild-type and mutant Paramecium tetraurelia ...
Eighty-two temperature-sensitive mutants of Paramecium tetraurelia that involve some aspect of endocytosis or vacuolar processing were isolated following nitrosoguanidine treatment. Two of these, the recessive mutations fvc (food vacuole clumping) and dml (defective membrane) , were examined in some...
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| Format: | Text |
| Language: | English |
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University of British Columbia
2010
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| Online Access: | https://dx.doi.org/10.14288/1.0095146 https://doi.library.ubc.ca/10.14288/1.0095146 |
| Summary: | Eighty-two temperature-sensitive mutants of Paramecium tetraurelia that involve some aspect of endocytosis or vacuolar processing were isolated following nitrosoguanidine treatment. Two of these, the recessive mutations fvc (food vacuole clumping) and dml (defective membrane) , were examined in some detail. At the restrictive temperature, 34.5°C, the food vacuoles of fvc cells clumped in one area of the cell, usually the posterior. Fewer food vacuoles accumulated in fvc cells than in wild-type cells after 20 minutes in a food vacuole marker, such as blue watercolor (BP). The fvc mutation was originally recovered as a double mutation and was linked by 34 ± 3 (standard deviation) map units to the tnd (temperature-sensitive, trichocyst non-discharge) locus. The mutant, dml, had morphologically abnormal vacuoles which, in the light microscope, appeared as a mass of fused or disrupted vacuoles. An ultrastructural study revealed that the structural integrity of the food vacuoles was lost. Abnormal mitochondria and ... |
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