Microbial diversity in soilless rhizospheres using potato dextrose agar and root inoculants ...

Microbiome diversities in soilless rhizospheres are becoming known as an important factor for urban agriculture and food security. Plant pathogens like Pythium are common in hydroponic systems, but symptoms can be prevented with an established rhizo-microbiome. This study explored the microbiome wit...

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Bibliographic Details
Main Authors: BRIANNA AKUAMOAH-BOATENG, SRIJA ALURI, CYNTHIA JOSHELIN SANCHEZ ARREGUIN, ALICIA LEE, MAEGAN MCBRIDE, ANSHU PALICHERLA, HARRY WANG, Nolan, Donielle
Format: Article in Journal/Newspaper
Language:English
Published: Journal of Student-Scientists' Research 2023
Subjects:
Online Access:https://dx.doi.org/10.13021/jssr2023.3936
https://journals.gmu.edu/index.php/jssr/article/view/3936
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Summary:Microbiome diversities in soilless rhizospheres are becoming known as an important factor for urban agriculture and food security. Plant pathogens like Pythium are common in hydroponic systems, but symptoms can be prevented with an established rhizo-microbiome. This study explored the microbiome within a single greenhouse to compare the dominant microorganisms in different systems and crops. Petri dishes were sterilized by soaking in 16% sodium hypochlorite and then isopropyl alcohol (70%). Potato dextrose agar (PDA) was inoculated with six samples; vermicompost castings, liquid mycorrhizal Orca (Plant Revolution Inc.), surface sterilized roots from a rotting Kalanchoe sp. in soil, an aquaponic Fragaria x ananassa, hydroponic Ocimum basilicum, and Lactuca sativa. After 5 days of incubation, the plates showed a few types of colonies (1-6 organisms per dish), with many single organisms consuming the entire dish. The vermicompost castings had the greatest number of species compared to the other samples ... : Journal of Student-Scientists' Research, Vol. 5 (2023) ...