The glycoprotein, non-virion protein, and polymerase of viral hemorrhagic septicemia virus are not determinants of host-specific virulence in rainbow trout ...

Background Viral hemorrhagic septicemia virus (VHSV), a fish rhabdovirus belonging to the Novirhabdovirus genus, causes severe disease and mortality in many marine and freshwater fish species worldwide. VHSV isolates are classified into four genotypes and each group is endemic to specific geographic...

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Bibliographic Details
Main Authors: Yusuff, Shamila, Kurath, Gael, Sun Kim, Min, Tesfaye, Tarin M., Li, Jie, McKenney, Douglas G., Vakharia, Vikram N.
Format: Article in Journal/Newspaper
Language:English
Published: BioMed Central Ltd 2019
Subjects:
viral hemorrhagic septicemia virus VHSV
virulence determinant
fish
rhabdovirus
glycoprotein
non-virion protein
polymerase protein
Pacific
North Atlantic
Online Access:https://dx.doi.org/10.13016/m2vpol-5ltb
http://mdsoar.org/handle/11603/13128
Description
Summary:Background Viral hemorrhagic septicemia virus (VHSV), a fish rhabdovirus belonging to the Novirhabdovirus genus, causes severe disease and mortality in many marine and freshwater fish species worldwide. VHSV isolates are classified into four genotypes and each group is endemic to specific geographic regions in the north Atlantic and Pacific Oceans. Most viruses in the European VHSV genotype Ia are highly virulent for rainbow trout (Oncorhynchus mykiss), whereas, VHSV genotype IVb viruses from the Great Lakes region in the United States, which caused high mortality in wild freshwater fish species, are avirulent for trout. This study describes molecular characterization and construction of an infectious clone of the virulent VHSV-Ia strain DK-3592B from Denmark, and application of the clone in reverse genetics to investigate the role of selected VHSV protein(s) in host-specific virulence in rainbow trout (referred to as trout-virulence). Methods Overlapping cDNA fragments of the DK-3592B genome were cloned ...

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