Data from: Isolation and characterization of 145 polymorphic microsatellite loci for the common frog (Rana temporaria)
We describe primers and polymerase chain reaction conditions to amplify 145 di-, tri- and tetranucleotide microsatellite loci from the common frog (Rana temporaria), a species commonly used as a model in ecological and evolutionary research. Primers were tested on 46 individuals from two Fennoscandi...
Main Authors: | , |
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Language: | unknown |
Published: |
2010
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Subjects: | |
Online Access: | http://nbn-resolving.org/urn:nbn:nl:ui:13-ds-nfvn https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:80510 |
Summary: | We describe primers and polymerase chain reaction conditions to amplify 145 di-, tri- and tetranucleotide microsatellite loci from the common frog (Rana temporaria), a species commonly used as a model in ecological and evolutionary research. Primers were tested on 46 individuals from two Fennoscandian populations and yielded an average of six to nine alleles per locus (range = 1–30) depending on the population. Average observed heterozygosities in the two populations were 0.16 (range = 0–0.91) and 0.36 (range = 0–1). |
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